The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies.
The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies.
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DOI：
10.4049/jimmunol.133.1.180
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发表时间：
1984-07
影响因子：
4.4
通讯作者：
B. Perussia;G. Trinchieri;A. Jackson;N. Warner;J. Faust;H. Rumpold;D. Kraft;L. Lanier
中科院分区：
文献类型：
--
作者：
B. Perussia;G. Trinchieri;A. Jackson;N. Warner;J. Faust;H. Rumpold;D. Kraft;L. Lanier
我们比较了与自然杀伤（NK）细胞和多形核细胞（PMN）反应的五种单克隆抗体（B73.1，3G 8，Leu-11 a，Leu- 11b和VEP 13）。我们表明，所有这些抗体都是针对和抑制IgG的Fc部分（FcR）的受体的功能特性。在与免疫复合物反应后NK细胞上FcR的调节诱导由五种抗体中的每一种识别的抗原的消失。相反，抗体阻断IgG致敏的红细胞与NK细胞和PMN的结合，并抑制它们介导针对抗体致敏的肿瘤靶细胞的细胞毒性的能力。通过使用双色免疫荧光技术，我们直接表征了被这些抗体识别的淋巴细胞群，并显示它是一个不携带B或T细胞标志物的同质亚群，除了20 - 50%的细胞中存在的抑制性/细胞毒性T细胞的33，000道尔顿抗原特征，以及45，绵羊红细胞的道尔顿受体存在于80 - 90%的细胞上。与单克隆抗体反应的细胞的表型对应于NK细胞的表型。交叉竞争实验表明，这些抗体检测FcR上至少两个不同的表位，一个（B73.1）优先在NK细胞上表达，一个或多个（3G 8/Leu-11 a/Leu- 11b /VEP 13）优先在PMN上表达。这些抗体与B细胞缺乏反应性表明人B细胞携带与NK细胞和PMN上不同的FcR。
We compare five monoclonal antibodies ( B73 .1, 3G8 , Leu- 11a , Leu- 11b , and VEP13 ) that react with natural killer (NK) cells and polymorphonuclear cells (PMN). We show that all of these antibodies are directed against and inhibit the functional properties of the receptor for the Fc portion of IgG (FcR). Modulation of the FcR on NK cells after reaction with immune complexes induces the disappearance of the antigen(s) recognized by each of the five antibodies. Conversely, the antibodies block binding of IgG-sensitized erythrocytes to the NK cells and PMN and inhibit their ability to mediate cytotoxicity against antibody-sensitized tumor target cells. By using two-color immunofluorescence techniques, we characterize directly the lymphocyte population recognized by these antibodies and show that it is a homogeneous subset that does not bear markers of either B or T cells, with the exception of the 33,000 dalton antigen characteristic of suppressor/cytotoxic T cells present in 20 to 50% of the cells, and the 45,000 dalton receptor for sheep erythrocytes present on 80 to 90% of the cells. The phenotype of the cells reacting with the monoclonal antibodies corresponds to that of NK cells. Cross-competition experiments indicate that these antibodies detect at least two distinct epitopes on FcR, one ( B73 .1) preferentially expressed on NK cells and one or more ( 3G8 /Leu- 11a /Leu- 11b / VEP13 ) preferentially expressed on PMN. The lack of reactivity of these antibodies with B cells suggests that human B cells bear a different FcR from that on NK cells and PMN.