Differential expression of the Na+-Ca2+ exchanger transcripts and proteins in rat brain regions
Differential expression of the Na+-Ca2+ exchanger transcripts and proteins in rat brain regions
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DOI：
10.1002/cne.10665
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发表时间：
2003-06-16
影响因子：
2.5
通讯作者：
Annunziato，L
中科院分区：
文献类型：
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作者：
Papa，M;Canitano，A;Annunziato，L
在中枢神经系统(CNS)中，Na+-Ca~(2+)交换器在控制细胞内Na~+和Ca~(2+)离子浓度的变化中起着基础性作用。众所周知，这些阳离子调节神经递质的释放、细胞的迁移和分化、基因表达和神经退化过程。本研究采用非放射性原位杂交和免疫组织化学的方法，对已知的三个钠-钙交换基因NCX1、NCX2和NCX3的转录本和蛋白的区域和细胞分布进行了研究。NCX1转录本在运动皮质的III-V层、丘脑、CA3和海马齿状回、下丘脑的几个核和小脑中都有特异的表达。NCX2转录本在海马区、纹状体和丘脑室旁核均有强表达。NCX3mRNAs主要在海马体、丘脑、杏仁核和小脑中表达。免疫组织化学分析显示，NCX1蛋白主要表达于大脑皮层颗粒上层、海马区、下丘脑、黑质和腹侧被盖区，以及小脑颗粒层。NCX2蛋白主要在海马体、纹状体、丘脑和下丘脑表达。NCX3蛋白主要分布于CA3区、海马区、放射层和腔隙分子层、腹侧纹状体和小脑分子层。总之，这些结果表明，不同的Na+-Ca~(2+)交换异构体似乎选择性地在几个中枢神经系统区域表达，在这些区域中，它们可能具有不同的功能作用。J.Comp.神经。461：31-48,2003。©2003 Wiley-Liss公司
In the central nervous system (CNS), the Na+‐Ca2+exchanger plays a fundamental role in controlling the changes in the intracellular concentrations of Na+and Ca2+ions. These cations are known to regulate neurotransmitter release, cell migration and differentiation, gene expression, and neurodegenerative processes. In the present study, nonradioactive in situ hybridization and light immunohistochemistry were carried out to map the regional and cellular distribution for both transcripts and proteins encoded by the three known Na+‐Ca2+exchanger genes NCX1, NCX2, and NCX3. NCX1 transcripts were particularly expressed in layers III‐V of the motor cortex, in the thalamus, in CA3 and the dentate gyrus of the hippocampus, in several hypothalamic nuclei, and in the cerebellum. NCX2 transcripts were strongly expressed in all hippocampal subregions, in the striatum, and in the paraventricular thalamic nucleus. NCX3 mRNAs were mainly detected in the hippocampus, in the thalamus, in the amygdala, and in the cerebellum. Immunohistochemical analysis revealed that NCX1 protein was mainly expressed in the supragranular layers of the cerebral cortex, in the hippocampus, in the hypothalamus, in the substantia nigra and ventral tegmental area, and in the granular layer of the cerebellum. The NCX2 protein was predominantly expressed in the hippocampus, in the striatum, in the thalamus, and in the hypothalamus. The NCX3 protein was particularly found in the CA3 subregion, and in the oriens, radiatum, and lacunoso‐moleculare layers of the hippocampus, in the ventral striatum, and in the cerebellar molecular layer. Collectively, these results suggest that the different Na+‐Ca2+exchanger isoforms appear to be selectively expressed in several CNS regions where they might underlie different functional roles. J. Comp. Neurol. 461:31–48, 2003. © 2003 Wiley‐Liss, Inc.