THE FAS PROTEIN IS EXPRESSED AT HIGH-LEVELS ON CD4+CD8+ THYMOCYTES AND ACTIVATED MATURE LYMPHOCYTES IN NORMAL MICE BUT NOT IN THE LUPUS-PRONE STRAIN, MRL LPR/LPR
THE FAS PROTEIN IS EXPRESSED AT HIGH-LEVELS ON CD4+CD8+ THYMOCYTES AND ACTIVATED MATURE LYMPHOCYTES IN NORMAL MICE BUT NOT IN THE LUPUS-PRONE STRAIN, MRL LPR/LPR
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DOI：
10.1073/pnas.90.21.10340
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发表时间：
1993-11-01
影响因子：
11.1
通讯作者：
ELKON, KB
中科院分区：
文献类型：
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作者：
DRAPPA, J;BROT, N;ELKON, KB
MRL品系小鼠的淋巴细胞增殖（lpr）突变是由Fas基因中插入早期转座因子ETn引起的。插入导致Fas mRNA表达的显著降低，并且在临床上与狼疮样疾病的显著加速相关。为了进一步探索Fas蛋白在胸腺中的T细胞选择和外周免疫系统中的耐受性中的作用，我们产生了与蛋白的多态性区域结合的单特异性多克隆抗鼠Fas抗体。Fas蛋白在BALB/c和MRL +/+胸腺细胞上的表达率几乎达到99%，且在大多数胸腺细胞因凋亡而死亡的CD 4 + CD 8+胸腺细胞上表达最高。与胸腺细胞上Fas的高水平表达相反，
The lymphoproliferation (lpr) mutation the MRL strain of mice is caused by the insertion of the early transposable element ETn in the Fas gene. The insertion causes a striking decrease in Fas mRNA expression and is associated clinically with marked acceleration of the lupus-like disease. To further explore the role of the Fas protein in T-cell selection in the thymus and tolerance in the peripheral immune system, we produced a monospecific polyclonal anti-murine Fas antibody that binds to a polymorphic region of the protein. Fas protein expression was detected on almost-equal-to 99% of BALB/c and MRL +/+ thymocytes, and the expression was highest on CD4+CD8+ thymocytes, the stage at which most thymocytes die by apoptosis. In contrast to the high level of expression of Fas on thymocytes, Fas was detected on