Enzymes immobilized on amine-terminated ionic liquid-functionalized carbon nanotube for hydrogen peroxide determination.
Enzymes immobilized on amine-terminated ionic liquid-functionalized carbon nanotube for hydrogen peroxide determination.
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DOI：
10.1016/j.talanta.2012.11.059
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发表时间：
2013-02
期刊：
影响因子：
6.1
通讯作者：
Xiuhui Liu;Caihong Bu;Zhihan Nan;Lichun Zheng;Yu Qiu;Xiaoquan Lu
中科院分区：
文献类型：
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作者：
Xiuhui Liu;Caihong Bu;Zhihan Nan;Lichun Zheng;Yu Qiu;Xiaoquan Lu
我们报告了一种基于细胞色素 C (Cyt c) 固定离子液体 (IL) 功能化多壁碳纳米管 (MWCNT) 修饰的玻璃碳电极 (GCE) 的过氧化氢 (H2O2) 电化学检测新方法。利用傅里叶变换红外光谱（FTIR）、紫外-可见光谱和电化学阻抗谱（EIS）对多壁碳纳米管与胺基封端离子液体材料的功能化进行了表征，结果表明，ILs对MWCNT的共价修饰表现出高的酶固定化表面积，并为Cyt c保留其对H2O2的生物电催化活性提供了良好的微环境。采用电流分析法评估细胞色素c对H2O2的催化活性。所提出的生物传感器表现出较H2O2接近4个数量级的宽线性响应范围(4.0×10−8M–1.0×10−4M)，具有良好的线性度(0.9980)和1.3×10−8M的低检测限(基于S/N=3)。此外，该生物传感器还表现出选择性高、重现性好、长期稳定性好等优异特性。因此，本研究构建的生物传感器在检测复杂生物系统中的H2O2方面具有巨大的潜力。
We report on a new approach for the electrochemical detection of hydrogen peroxide (H2O2) based on Cytochrome C (Cyt c) immobilized ionic liquid (IL)-functionalized multi-walled carbon nanotubes (MWCNTs) modified glass carbon electrode (GCE). Functionalization of multi-walled carbon nanotube with amine-terminated ionic liquid materials was characterized using fourier transform infrared spectroscopy (FTIR), UV–vis spectra, and electrochemical impedance spectroscopy (EIS), and the results showed that the covalent modification of MWCNTs with ILs exhibited a high surface area for enzyme immobilization and provided a good microenvironment for Cyt c to retain its bioelectrocatalytic activity toward H2O2. Amperometry was used to evaluate the catalytic activity of the cyt c towards H2O2.The proposed biosensor exhibited a wide linear response range nearly 4 orders of magnitude of H2O2(4.0×10−8M–1.0×10−4M) with a good linearity (0.9980) and a low detection limit of 1.3×10−8M (based on S/N=3). Furthermore, the biosensor also displays some other excellent characteristics such as high selectivity, good reproducibility and long-term stability. Thus, the biosensor constructed in this study has great potential for detecting H2O2in the complex biosystems.