HYDROLYSIS OF DIETARY FLAVONOID GLYCOSIDES BY STRAINS OF INTESTINAL BACTEROIDES FROM HUMANS
HYDROLYSIS OF DIETARY FLAVONOID GLYCOSIDES BY STRAINS OF INTESTINAL BACTEROIDES FROM HUMANS
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DOI：
10.1042/bj2480953
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发表时间：
1987-12-15
影响因子：
4.1
通讯作者：
WINTER, J
中科院分区：
文献类型：
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作者：
BOKKENHEUSER, VD;SHACKLETON, CHL;WINTER, J
芦丁和槲皮苷被健康受试者的粪便菌群水解为槲皮素，知更鸟素被水解为山奈酚。这些水解所需的酶，即α-鼠李糖苷酶和β-半乳糖苷酶，是由Bacteroides distasonis的一些菌株产生的；然而，其他菌株合成了β-葡萄糖苷酶。最后命名的酶也是由统一拟杆菌和卵形拟杆菌精心设计的。所有酶都是组成型产生的。含有β-葡萄糖苷酶的B. distasonis无细胞提取物表现出每10mg蛋白质1μmol/10分钟的酶活性。
Rutin and quercitrin are hydrolysed to quercetin, and robinin is hydrolysed to kaempferol, by faecal flora from healthy subjects. The enzymes required for these hydrolyses, namely .alpha.-rhamnosidase and .beta.-galactosidase, were produced by some strains of Bacteroides distasonis; other strains, however, synthesized .beta.-glucosidase. The last-named enzyme was also elaborated by Bacteroides uniformis and Bacteroides ovatus. All the enzymes were produced constitutively. A cell-free extract of B. distasonis containing .beta.-glucosidase displayed an enzymic activity of 1 .mu.mol/10 min per 10 mg of protein.