EVALUATION OF THE MUTAGENIC EFFECTS OF SV40 IN MOUSE, HAMSTER, AND MOUSE-HUMAN HYBRID-CELLS
EVALUATION OF THE MUTAGENIC EFFECTS OF SV40 IN MOUSE, HAMSTER, AND MOUSE-HUMAN HYBRID-CELLS
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DOI：
10.1007/bf01233058
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发表时间：
1991-07-01
期刊：
影响因子：
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通讯作者：
BROCKMAN, WW
中科院分区：
文献类型：
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作者：
GILES, RE;BOYCE, FM;BROCKMAN, WW
我们研究了SV 40在小鼠、仓鼠和小鼠-人杂交细胞中诱导耐药性或耐温性变化的能力。 SV 40诱导Balb/c 3 T3细胞对5-溴脱氧尿苷+三氟胸苷的耐药细胞显著增加，并诱导对6-硫鸟嘌呤的耐药杂交细胞增加。 在其他测试系统中发现SV 40无致突变性或弱致突变性。 3 T3细胞呈T抗原阳性，TK活性显著降低，放射自显影显示[H-3]BrdU掺入异质，耐药表型不稳定，表明SV 40可能通过表观遗传过程诱导耐药。 SV 40诱导的6-硫代鸟嘌呤抗性的杂交似乎主要发生染色体丢失。
We have examined the ability of SV40 to induce changes in drug or temperature resistance in mouse, hamster, and mouse-human hybrid cells. SV40 induced a substantial increase of cells resistant to 5-bromodeoxyuridine + trifluorothymidine in Balb/c 3T3 cells and induced an increase of hybrid cells resistant to 6-thioguanine. SV40 was found to be nonmutagenic or weakly mutagenic in other test systems. The 3T3 cells were T-antigen positive, exhibited a marked reduction in TK activity, were heterogeneous for [H-3]BrdU incorporation by autoradiography, and exhibited instability of the drug-resistance phenotype, suggesting that SV40 may be inducing resistance by an epigenetic process. SV40-induced 6-thioguanine resistance in the hybrids appears to occur predominantly by chromosome loss.