Organization of the mouse macrophage C-type lectin,(Mcl) gene and identification of a subgroup of related lectin molecules
Organization of the mouse macrophage C-type lectin,(Mcl) gene and identification of a subgroup of related lectin molecules
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DOI：
10.1046/j.1365-2370.2002.00266.x
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发表时间：
2002-02-01
期刊：
影响因子：
--
通讯作者：
McKnight, AJ
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文献类型：
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作者：
Balch, SG;Greaves, DR;McKnight, AJ
许多编码c型凝集素分子的基因已被定位到小鼠6号染色体远端区域的自然杀伤基因复合体（NKC）和人类12p12-p13染色体的一个合成区域。除了那些调节NK细胞功能的受体外，在其他细胞类型上表达的相关结构也定位于该染色体区域。其中有一些最近被发现的基因，包括巨噬细胞c型凝集素（MCL）、巨噬细胞诱导的c型凝集素（Mincle）、树突状细胞免疫受体（DCIR）和树突状细胞相关的凝集素-2 （Dectin-2）。由MCL、Mincle、DCIR和Dectin-2的单c型凝集素结构域组成的氨基酸序列彼此密切相关。这些分子总体上与动物G型凝集素II和V组相似，表现出II型跨膜拓扑结构。在本研究中，序列分析表明MCL、Mincle、DCIR和Dectin-2是ii组相关c型凝集素的一个亚群，可能参与巨噬细胞和树突状细胞类似的识别事件。MCL的基因组组织。从小鼠MCL中测定了基因和启动子区序列，并推测了调控元件。基因组DNA克隆和在这里详细描述。
A number of genes encoding C-type lectin molecules have been mapped to the natural killer gene complex (NKC) at the distal region of mouse chromosome 6 and to a syntenic region on human chromosome 12p12-p13. In addition to those receptors which regulate NK cell function, related structures expressed on other cells types have also been localized to this chromosomal region. Among these are a number of recently characterized genes, including macrophage C-type lectin (MCL), macrophage-inducible C-type lectin (Mincle), dendritic cell immunoreceptor (DCIR) and dendritic cell-associated lectin-2 (Dectin-2). The amino acid sequences comprising the single C-type lectin domains of MCL, Mincle, DCIR and Dectin-2 are shown here to be closely related to each other. These molecules show overall similarity to two groups of animal G type lectins, groups II and V, which demonstrate type II transmembrane topology. In this study, sequence analysis suggests that MCL, Mincle, DCIR and Dectin-2 represent a subset of group II-related C-type lectins which may participate in analogous recognition events on macrophages and dendritic cells. The genomic organization of the MCL. gene and the sequence of the promoter region, with putative regulatory elements, were determined from a mouse MCL. genomic DNA clone and are described here in detail.