HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF NANOMOLE LEVELS OF GLUTATHIONE, GLUTATHIONE DISULFIDE, AND RELATED THIOLS AND DISULFIDES
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF NANOMOLE LEVELS OF GLUTATHIONE, GLUTATHIONE DISULFIDE, AND RELATED THIOLS AND DISULFIDES
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DOI：
10.1016/0003-2697(80)90118-9
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
POTTER, DW
中科院分区：
文献类型：
--
作者：
REED, DJ;BABSON, JR;POTTER, DW
建立了测定纳摩尔级谷胱甘肽、谷胱甘肽二硫化物、半胱氨酸谷胱甘肽混合二硫化物及20种相关含硫氨基酸及其衍生物的高效液相色谱法。该方法是基于游离硫醇与碘乙酸的S-羧甲基衍生物的初始形成，然后通过与1-氟-2，4-二硝基苯反应将游离氨基转化为2，4-二硝基苯基衍生物。在3-氨基丙基硅烷衍生的硅胶柱上对反应混合物进行色谱分析，不进行样品分离，用乙酸钠或乙酸铵梯度在pH 4.5的水-甲醇-乙酸溶剂中洗脱。描述了生物样品[大鼠肝细胞]中谷胱甘肽、谷胱甘肽二硫化物和半胱氨酸谷胱甘肽混合二硫化物纳摩尔水平的测定。
A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20 related sulfur-containing amino acids or their derivatives was described. The procedure is based upon the initial formation of S-carboxymethyl derivatives of free thiols with iodoacetic acid followed by conversion of free amino groups to 2,4-dinitrophenyl derivatives by reaction with 1-fluoro-2,4-dinitrobenzene. Chromatography of the reaction mixture without sample isolation is on a 3-aminopropylsilane derivatized silica column and elution with a sodium or ammonium acetate gradient in a water-methanol-acetic acid solvent at pH 4.5. Determination of nanomole levels of glutathione, glutathione disulfide and cysteine glutathione-mixed disulfide in biological samples [rat hepatocytes] is described.