Clip compression model is useful for thoracic spinal cord injuries - Histologic and functional correlates
Clip compression model is useful for thoracic spinal cord injuries - Histologic and functional correlates
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DOI：
10.1097/brs.0b013e31815b7e6b
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发表时间：
2007-12-01
期刊：
影响因子：
3
通讯作者：
Tator, Charles H.
中科院分区：
文献类型：
--
作者：
Poon, Peter C.;Gupta, Dimpy;Tator, Charles H.
研究设计.大鼠急性胸段脊髓损伤模型的实验研究，包括模拟人类损伤的急性夹压。评估该模型的剂量反应，以了解大鼠胸脊髓损伤力与组织学和功能结果测量之间的关系。急性硬膜外夹压损伤已成为一种可靠的模型，用于产生急性实验性颈脊髓损伤，然而，该模型尚未正式评估急性胸脊髓损伤的剂量-反应曲线。在Sprague-Dawley大鼠T2椎板切除术后，在T2脊髓周围硬膜外应用施加20、26或35 g闭合力的改良动脉瘤夹，然后快速释放，脊髓压迫持续1分钟。选择这些力分别模拟轻度至中度、中度和中度至重度的急性压迫性损伤（n = 8/组）。每周用Basso、Beattie和Bresnahan（BBB）旷场运动试验评估运动活动，持续4周。然后对损伤的脊髓进行组织学检查，包括空洞的定量。观察到夹持力和BBB评分的显著主效应（F（2，20）= 5.42，P = 0.013）。损伤后4周，20 g和35 g夹伤组的BBB评分有显著差异（P = 0.05）。4周时的空洞体积与损伤程度成正比，20 g组的空洞体积明显小于35 g组（P <0. 05），空洞体积与BBB评分相关。大鼠胸髓夹压迫模型是一种可重复的、临床相关的脊髓损伤模型。这是第一次在大鼠胸髓的夹压损伤的力量与功能和组织学结果的措施。
Study Design. Experimental investigation of an acute thoracic spinal cord injury model in rats involving acute clip compression that simulates human injury.Objective. To assess the dose-response of this model for the relationship between the force of injury on the rat thoracic spinal cord and histological and functional outcome measures.Summary of Background Data. Acute extradural clip compression injury has been a reliable model for producing acute experimental cervical spinal cord injury; however, this model has not been formally evaluated with dose-response curves for acute injury of the thoracic spinal cord.Methods. After laminectomy at T2 in Sprague-Dawley rats, a modified aneurysm clip exerting a closing force of 20, 26, or 35 g was applied extradurally around the spinal cord at T2, and then rapidly released with cord compression persisting for 1 minute. These forces were selected to simulate acute compression injuries of mild to moderate, moderate, and moderate to severe degrees, respectively (n = 8/group). Motor activity was assessed weekly for 4 weeks with the Basso, Beattie, and Bresnahan (BBB) open field locomotor test. The injured spinal cord was then examined histologically including quantification of cavitation.Results. A significant main effect was observed for clip force and BBB score ( F( 2,20) = 5.42, P = 0.013). For 4 weeks after injury, the BBB scores for the 20 g and 35 g clip injury groups were significantly different ( P = 0.05). The cavitation volume at 4 weeks was directly proportional to the severity of injury: the 20 g group had significantly smaller cavities than the 35 g group ( P < 0.05), and the cavitation volume correlated with the BBB scores.Conclusion. The rat thoracic cord clip compression model is a reproducible, clinically relevant spinal cord injury model. This is the first time that the force of clip compression injury in the rat thoracic cord has been correlated with both functional and histologic outcome measures.