Genetic variation of Porphyromonas gingivalis genes encoding gingipains, cysteine proteinases with arginine or lysine specificity
Genetic variation of Porphyromonas gingivalis genes encoding gingipains, cysteine proteinases with arginine or lysine specificity
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DOI：
10.1515/bchm.1998.379.2.205
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发表时间：
1998-02-01
影响因子：
3.7
通讯作者：
Potempa, J
中科院分区：
文献类型：
--
作者：
Mikolajczyk-Pawlinska, J;Kordula, T;Potempa, J
牙龈卟啉单胞菌血凝半胱氨酸蛋白酶（牙龈卟啉菌蛋白酶）被认为是成人牙周炎发生发展的重要毒力因子。使用Southern印迹分析，确定了三种不同的基因：但相关的牙龈卟啉酶（牙龈卟啉菌蛋白酶-R1、牙龈卟啉菌蛋白酶-R2和牙龈卟啉菌蛋白酶-K）存在于所有测试菌株中，包括HG 66、ATCC 33277、W50、E-20-1、EM-3、381、A7436和IKG 5，其中编码牙龈卟啉菌蛋白酶-R1和牙龈卟啉菌蛋白酶-K的血凝素结构域的一部分的区域显示出相当大的变异性。与此相反，基因座编码牙龈卟啉菌蛋白酶-R1（rgp 1）和牙龈卟啉菌蛋白酶-R2（rgp 2）的强烈保守排除并发发生的其他牙龈卟啉菌蛋白酶-R-样基因，如cpgR和agp。值得注意的是，没有证据可以被发现支持的基因编码的推定的蛋白酶卟啉蛋白酶，一种酶的表达，建议有牙龈卟啉蛋白酶R和牙龈卟啉蛋白酶K的活动。
Porphyromonas gingivalis hemagglutinating cysteine proteinases (gingipains) are considered as important virulence factors in the development of adult periodontitis. Using Southern blot analysis it was determined that genes of three distinct: but related gingipains (gingipain-R1, gingipain-R2 and gingipain-K) were present in all tested strains including HG66, ATCC 33277, W50, E-20-1, EM-3, 381, A7436, and IKG5, with a region encoding a part of the hemagglutinin domain of gingipain-R1 and gingipain-K showing considerable variability. In contrast, the loci encoding gingipain-R1 (rgp1) and gingipain-R2 (rgp2) were strongly conserved excluding the concurrent occurrence of other gingipain-R-like genes such as cpgR and agp. Significantly, no evidence could be found to support the expression of a gene coding for the putative proteinase porphypain, an enzyme suggested to have both gingipain-R and gingipain-K activity.