Immunoprecipitation of melanogenic enzyme autoantigens with vitiligo sera: Evidence for cross-reactive autoantibodies to tyrosinase and tyrosinase-related protein-2 (TRP-2)
Immunoprecipitation of melanogenic enzyme autoantigens with vitiligo sera: Evidence for cross-reactive autoantibodies to tyrosinase and tyrosinase-related protein-2 (TRP-2)
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DOI：
10.1046/j.1365-2249.1997.4781381.x
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发表时间：
1997-09-01
影响因子：
4.6
通讯作者：
Weetman, AP
中科院分区：
文献类型：
--
作者：
Kemp, EH;Gawkrodger, DJ;Weetman, AP
在本研究中，我们描述了使用体外s -35标记的人TRP-2放射免疫分析法检测白癜风患者的TRP-2抗体。在检测的53份白癜风血清中，有3份（5.9%）发现TRP-2抗体阳性。20例对照血清、10例桥本甲状腺炎患者血清和10例Graves病患者血清均为阴性。3例TRP-2抗体阳性患者（平均年龄54岁，年龄50-63岁）均患有对称型白癜风1年以上，并伴有相关自身免疫性疾病：1例Graves病，2例自身免疫性甲状腺功能减退。此外，血清中还存在黑素生成酶酪氨酸酶的抗体。为了检验TRP-2和酪氨酸酶之间的免疫交叉反应性，我们用含有表达TRP-2或酪氨酸酶的COS-7细胞提取物预先吸收了3例TRP-2抗体阳性的白癜风血清，随后用于放射免疫测定。这些吸收研究表明，在体外翻译的S-35-TRP-2免疫测定中，这两种蛋白的预孵育抑制了阳性血清的免疫反应性。这种抗体的交叉反应性表明，这些患者对这两种黑色素生成酶的体液反应可能不是完全独立的。
In the present study we describe the detection of TRP-2 antibodies in vitiligo patients using in vitro S-35-labelled human TRP-2 in a radioimmunoassay. Of 53 vitiligo sera examined in the assay, three (5.9%) were found to be positive for TRP-2 antibodies. In contrast, 20 control sera, sera from 10 patients with Hashimoto's thyroiditis and sera from 10 patients with Graves' disease were all negative. All three patients positive for TRP-2 antibodies (mean age 54 years, age range 50-63 years) had had vitiligo of the symmetrical type for more than 1 year and all of them also had an associated autoimmune disorder: Graves' disease in one and autoimmune hypothyroidism in two. In addition, antibodies to the melanogenic enzyme tyrosinase were present in their serum. To examine any immunological crossreactivity between TRP-2 and tyrosinase, the three vitiligo sera positive for TRP-2 antibodies were preabsorbed with COS-7 cell extract containing either expressed TRP-2 or tyrosinase, and subsequently used in the radioimmunoassay. These absorption studies indicated that preincubation with both proteins inhibited the immunoreactivity of the positive sera in the immunoassay using in vitro translated S-35-TRP-2. This antibody cross-reactivity suggests the humoral response to the two melanogenic enzymes in these patients may not be entirely independent.