Multiwalled Carbon Nanotube for One-Step Cleanup of 21 Mycotoxins in Corn and Wheat Prior to Ultraperformance Liquid Chromatography⁻Tandem Mass Spectrometry Analysis.
Multiwalled Carbon Nanotube for One-Step Cleanup of 21 Mycotoxins in Corn and Wheat Prior to Ultraperformance Liquid Chromatography⁻Tandem Mass Spectrometry Analysis.
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在超高效液相色谱-串联质谱分析之前，使用多壁碳纳米管一步净化玉米和小麦中的 21 种霉菌毒素
DOI：
10.3390/toxins10100409
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发表时间：
2018-10-10
期刊：
影响因子：
4.2
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Jiang D;Wei D;Wang L;Ma S;Du Y;Wang M
建立了多壁碳纳米管（MWCNT）一步固相萃取（SPE）同时分析玉米和小麦中9种青霉烯、玉米赤霉烯酮（ZEN）及其衍生物、4种黄曲霉毒素和2种赭曲霉毒素等21种真菌毒素的方法。考察了多壁碳纳米管的种类、多壁碳纳米管与5种吸附剂（十八烷基硅烷（C18）、亲水亲油平衡（HLB）、混合阳离子交换（MCX）、硅胶和氨丙基（NH 2））的组合以及多壁碳纳米管的填充量对一步固相萃取性能的影响。20 mg羧基MWCNT和200 mg C18的组合被证明是最有效的，允许量化玉米和小麦中所有分析的真菌毒素。在超高效液相色谱-串联质谱（UPLC-MS/MS）分析前的优化净化程序下，通过分析以定量限（LOQ）、2倍LOQ和10倍LOQ加标的样品对该方法进行了验证。线性关系良好（r2 ≥ 0.9910），灵敏度高（定量限在0.5-25 μg L−1范围内），回收率高（75.6-110.3%），精密度好（相对标准偏差（RSD）为0.3-10.7%）。该方法的适用性进一步证实了使用玉米和小麦的原始样品。该方法快速、简便、可靠，可用于玉米和小麦中21种真菌毒素的同时检测。
One-step solid-phase extraction (SPE) using a multiwalled carbon nanotube (MWCNT) for simultaneous analysis of 21 mycotoxins, including nine trichothecenes, zearalenone (ZEN) and its derivatives, four aflatoxins, and two ochratoxins, in corn and wheat was developed. Several key parameters affecting the performance of the one-step SPE procedure—types of MWCNT, combinations with five sorbents (octadecylsilyl (C18), hydrophilic–lipophilic balance (HLB), mixed-mode cationic exchange (MCX), silica gel, and amino-propyl (NH2)), and filling amounts of the MWCNTs—were thoroughly investigated. The combination of 20 mg carboxylic MWCNT and 200 mg C18 was proven to be the most effective, allowing the quantification of all analyzed mycotoxins in corn and wheat. Under the optimized cleanup procedure prior to ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) analysis, the method was validated by analyzing samples spiked at the limit of quantification (LOQ), two-times LOQ, and 10-times LOQ. Satisfactory linearity (r2 ≥ 0.9910), high sensitivity (LOQ in different ranges of 0.5–25 μg L−1), good recovery (75.6–110.3%), and acceptable precision (relative standard deviation (RSD), 0.3–10.7%) were obtained. The applicability of the method was further confirmed using raw samples of corn and wheat. In conclusion, the established method was rapid, simple and reliable for simultaneous analysis of 21 mycotoxins in corn and wheat.