Aescin reduces oxidative stress and provides neuroprotection in experimental traumatic spinal cord injury
Aescin reduces oxidative stress and provides neuroprotection in experimental traumatic spinal cord injury
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DOI：
10.1016/j.freeradbiomed.2016.09.002
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发表时间：
2016-10-01
影响因子：
7.4
通讯作者：
Ju, Gong
中科院分区：
文献类型：
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作者：
Cheng, Peng;Kuang, Fang;Ju, Gong
七叶皂苷具有许多与脊髓损伤（SCI）高度相关的生理功能，包括抗炎、抗氧化、抗水肿、增强血管张力等。本研究调查了七叶皂苷在 SCI 中的假定治疗价值，重点关注其神经保护、抗炎和抗氧化特性。大鼠损伤后30 min静脉注射七叶皂苷钠（1.0 mg/kg体重）或等体积的生理盐水，中度SCI后每日追加剂量，连续7天。在第 8 胸椎 (T8) 脊髓挫伤后，经七叶皂苷治疗的大鼠后肢无力的严重程度低于盐水对照组，通过 Basso-Beattie-Bresnahan 量表、平衡木步行测试和足迹分析进行了测定。在撞击后的 T8 左右测量，七叶皂苷治疗的大鼠运动结果的改善与免疫反应、氧化应激、神经元损失、轴突脱髓鞘、脊髓肿胀和细胞凋亡显着降低相对应。我们的数据表明七叶皂苷治疗是 SCI 中一种新颖的早期神经保护方法。鉴于七叶皂苷在临床应用中已知的安全性，本研究的结果表明它是人类 SCI 治疗的良好候选者。 (C) 2016 Elsevier Inc. 保留所有权利。
Aescin has many physiological functions that are highly relevant to spinal cord injury (SCI), including anti-inflammation, anti-oxidation, anti-oedema, and enhancing vascular tone. The present study investigated the putative therapeutic value of aescin in SCI, with a focus on its neuroprotective, anti-inflammatory, and anti-oxidative properties. Sodium aescinate (1.0 mg/kg body weight) or equivalent volume of saline was administered 30 min after injury by intravenous injection, with an additional dose daily for seven consecutive days after moderate SCI in rats. After contusion injury of the 8th thoracic (T8) spinal cord, aescin-treated rats developed less severe hind limb weakness than saline controls, as assayed by the Basso-Beattie-Bresnahan scale, the beam walking test, and a footprint analysis. The improved locomotor outcomes in aescin-treated rats corresponded to markedly decreased immune response, oxidative stress, neuronal loss, axon demyelination, spinal cord swelling, and cell apoptosis, measured around T8 after impact. Our data suggest aescin treatment as a novel, early, neuroprotective approach in SCI. Given the known safety of aescin in clinical applications, the results of this study suggest that it is a good candidate for SCI treatment in humans. (C) 2016 Elsevier Inc. All rights reserved.