Morphological and genetic diversity analysis among three populations of clam Lutraria siebaldii in coastal Guangxi and Guangdong provinces
Morphological and genetic diversity analysis among three populations of clam Lutraria siebaldii in coastal Guangxi and Guangdong provinces
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发表时间：
2011
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通讯作者：
Qin Zhi-biao
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作者：
Qin Zhi-biao
采用随机扩增多态DNA（RAPD）技术和形态学变异分析，对广西沿海和广东沿海的3个大花蛤野生群体和越南沿海的1个大花蛤群体的145个样品进行了RAPD分析，共扩增出428个可标记，扩增片段大小在125 ~ 5000 bp之间，每个引物扩增出的条带数在6 ~ 17条之间，4个群体的平均多态位点百分率为76.1%~ 91.9个体间的遗传距离为0.1321 ~ 0.2441，根据遗传距离进行聚类分析，首先将湛江、北海和防城群体与越南群体进行聚类，结果表明，8个引物可区分3个野生居群，而湛江、北海和防城居群的样品在形态上比较相似，主成分分析结果表明，三个主成分的贡献率分别为49.13%、17.18%和14.02%，其中越南种群的贡献率最高，其次为越南种群，越南种群的贡献率最低，越南种群的贡献率最高，越南种群的贡献率最低，越南种群的贡献率最低，越南种群的贡献率最高，越南种群的贡献率最低，累积贡献率为80.33%。建立了4个人群的判别函数，P1和P2的判别准确率分别为88.9%~100.0%和86.7%~ 100.0%，综合判别准确率为95.9%，说明该种资源状况良好，遗传变异较大，具有良好的养殖前景。
The random amplified polymorphic DNA(RAPD) technique and the morphological variation analysis were used to evaluate population genetics among three wild populations of clam Lutraria siebaldii in coastal Guangxi and Guangdong provinces and one population Lutraria maxima in coastal Vietnam.A total of 428 scorable markers were amplified from 20 RAPD primers in 145 samples from different populations of Lutraria siebaldii and Lutraria maxima,ranging from 125 to 5 000 bp in size,and the number of the scorable bands for each primer varied from 6 to 17,with the average polymorphic band proportion of 84.5%.The mean percent of polymorphic loci in four populations were ranged from 76.1% to 91.9% with genetic distance from 0.1321 to 0.2441 among individuals.The cluster analysis according to their genetic distances,first Zhanjiang,Beihai and Fangcheng populations,and then with Vietnam population,revealed that the 3 wild populations of Lutraria siebaldii were distinguished by 8 primers.The morphological variations showed that the samples from Zhanjiang,Beihai and Fangcheng populations were rather similar in morphology,whereas Vietnam population differed greatly from the other populations in morphology,indicating that these clams were divided into different species.In the principal component analysis,the contributory ratio of the three principal components was 49.13%,17.18% and 14.02%,respectively,and the cumulative contributory ratio was 80.33%.The discrimination functions of the four populations were set up,the discrimination accuracy being 88.9%~100.0% for P1 and 86.7%~ 100.0% for P2,the integrative discrimination accuracy being 95.9%.The resource of Lutraria siebaldii was found in good condition with high genetic variation and Lutraria siebaldii had good culture prospects.