Analysis of GT-3a identifies a distinct subgroup of trihelix DNA-binding transcription factors in Arabidopsis
Analysis of GT-3a identifies a distinct subgroup of trihelix DNA-binding transcription factors in Arabidopsis
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DOI：
10.1016/s0014-5793(04)00222-4
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发表时间：
2004-03-26
期刊：
影响因子：
3.5
通讯作者：
Zhou, DX
中科院分区：
文献类型：
--
作者：
Ayadi, M;Delaporte, V;Zhou, DX
三螺旋DNA结合因子(或GT因子)与许多植物基因启动子中的GT元件结合。虽然一些成员(如GT-1和GT-2)的结合特异性和转录活性已经被研究，但这个转录因子家族的调节功能仍然很大程度上是未知的。在这项工作中，我们表征了一个新的GT因子，即GT-3a，以及一个密切相关的成员，GT-3b。我们发现：(4)它们可以形成同源或异源二聚体，但不与GT-1相互作用；(2)它们主要在花芽和根中表达；(3)GT-3a不能与GT-1或GT-2的结合部位结合，但通过5‘-GTAC序列与CAB2和RBCS-1A基因启动子结合，该序列已被证明是第1类GT元件的核心。这些结果表明，GT-3a和GT-3b属于不同的GT因子亚群，每个GT因子亚群都与不同类型的顺式作用GT元件结合。(C)2004年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Trihelix DNA-binding factors (or GT factors) bind to GT elements found in the promoters of many plant genes. Although the binding specificity and the transcriptional activity of some members (e.g. GT-1 and GT-2) have been studied, the regulatory function of this family of transcription factors remains largely unknown. In this work, we have characterised a new GT factor, namely GT-3a, and a closely related member, GT-3b. We show that (4) they can form either homo- or heterodimers but do not interact with GT-1; (2) they are predominantly expressed in floral buds and roots; (3) GT-3a cannot bind to the binding sites of GT-1 or GT-2, but binds to the cab2 and rbcS-1A gene promoters via the 5'-GTTAC sequence, which has been previously shown to be the core of the Site 1 type of GT elements. These results suggest that GT-3a and GT-3b belong to a distinct subgroup of GT factors and that each subgroup of GT factors binds to a functionally distinct type of cis-acting GT elements. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.