Outer nuclear membrane fusion of adjacent nuclei in varicella-zoster virus-induced syncytia.
Outer nuclear membrane fusion of adjacent nuclei in varicella-zoster virus-induced syncytia.
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DOI：
10.1016/j.virol.2017.09.002
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发表时间：
2017-12
期刊：
影响因子：
3.7
通讯作者：
Wei Wang;Lianwei Yang;Xiumin Huang;Wenkun Fu;D. Pan;Linli Cai;Jianghui Ye;Jian Liu;N. Xia;T. Cheng;Hua Zhu
中科院分区：
文献类型：
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作者：
Wei Wang;Lianwei Yang;Xiumin Huang;Wenkun Fu;D. Pan;Linli Cai;Jianghui Ye;Jian Liu;N. Xia;T. Cheng;Hua Zhu
合胞体形成被认为对于许多病毒的细胞间传播和发病机制很重要。当合胞体形成时，单个核经常聚集在一起，从而允许核膜紧密接触并可能发生融合。然而，目前还没有野生型病毒诱导的合胞体中相邻核之间核膜融合的证据报道。水痘带状疱疹病毒（VZV）是一种典型的合胞体诱导病毒，可引起人类水痘和带状疱疹。在这里，我们首次报道了在体外 ARPE-19 人上皮细胞和 SCID-hu 小鼠模型体内人皮肤异种移植物中包含 VZV 合胞体的并列核的外核膜明显融合的有趣观察。这项工作揭示了多核合胞体背景下 VZV 相关细胞病变效应的一个新方面。此外，这项研究提供的信息可能有助于未来研究病毒与宿主细胞核相互作用。
Syncytia formation has been considered important for cell-to-cell spread and pathogenesis of many viruses. As a syncytium forms, individual nuclei often congregate together, allowing close contact of nuclear membranes and possibly fusion to occur. However, there is currently no reported evidence of nuclear membrane fusion between adjacent nuclei in wild-type virus-induced syncytia. Varicella-zoster virus (VZV) is one typical syncytia-inducing virus that causes chickenpox and shingles in humans. Here, we report, for the first time, an interesting observation of apparent fusion of the outer nuclear membranes from juxtaposed nuclei that comprise VZV syncytia both in ARPE-19 human epithelial cellsin vitroand in human skin xenografts in the SCID-hu mouse modelin vivo. This work reveals a novel aspect of VZV-related cytopathic effect in the context of multinucleated syncytia. Additionally, the information provided by this study could be helpful for future studies on interactions of viruses with host cell nuclei.