DNA copy number changes in thyroid carcinoma
DNA copy number changes in thyroid carcinoma
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DOI：
10.1016/s0002-9440(10)65407-7
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发表时间：
1999-05-01
影响因子：
6
通讯作者：
Joensuu, H
中科院分区：
文献类型：
--
作者：
Hemmer, S;Wasenius, VM;Joensuu, H
我们应用比较基因组杂交技术（CGH）研究了69例原发性甲状腺癌的DNA拷贝数变化，在乳头状癌中，DNA拷贝数变化很少（3/26，12%），变化均为增益，与年龄（P = 0.01）和有无颈淋巴结转移（P = 0.08）有关。DNA拷贝数变化在滤泡癌中的发生率（16/20，80%）明显高于乳头状癌（P < 0.0001），滤泡癌中DNA拷贝数缺失的发生率（13/20 vs 0/26，P < 0.0001）也明显高于乳头状癌（P < 0.0001）。22号染色体丢失在滤泡性癌中常见（n = 7，35%），在广泛浸润性滤泡性癌中更常见（54%比0%，P = 0.04），并且与老年相关（P = 0.01）。4例滤泡癌中3例22号染色体丢失，10例髓样癌中5例（50%）DNA拷贝数改变。13例未分化癌中有11例（85%）出现DNA拷贝数改变，其中5例缺失，1例22号染色体缺失。未分化癌中最常见的DNA拷贝数改变发生在7 p（p22-pter，31%）、8q（q22-qter，23%）和9q（q34-qter，23%）。我们的结论是，DNA拷贝数的变化是经常在滤泡状癌，髓样癌和间变性甲状腺癌，但很少在乳头状癌的CGH研究时，22号染色体的丢失是特别常见的滤泡状癌，它是与广泛的浸润型。
The genetic changes leading to thyroid cancer are poorly characterized, We studied DNA copy number changes by comparative genomic hybridization (CGH) in 69 primary thyroid carcinomas, In papillary carcinoma, DNA copy number changes were rare (3 of 26, 12%), The changes were all gains, and they were associated with old age (P = 0.01) and the presence of cervical lymph node metastases at presentation (P = 0.08). DNA copy number changes were much more frequent in follicular carcinoma (16 of 20, 80%) than in papillary carcinoma (P < 0.0001), and follicular carcinomas had more often deletions (13/20 versus 0/26, P < 0.0001). Loss of chromosome 22 was common in follicular carcinoma (n = 7, 35%), it was more often seen in widely invasive than in minimally invasive follicular carcinoma (54% versus 0%, P = 0.04), and it was associated with old age at presentation (P = 0.01). In three of the four patients with follicular carcinoma who died of cancer, the tumor had loss of chromosome 22, DNA copy number changes were found in 5 (50%) of the 10 medullary carcinomas studied. Four of these five carcinomas had deletions, and in two of them there was deletion of chromosome 22, Eleven (85%) of the thirteen anaplastic carcinomas investigated had DNA copy number changes, of which five had deletions, and one had deletion of chromosome 22, The most common gains in anaplastic carcinoma were in chromosomes 7p (p22-pter, 31%), 8q (q22-qter, 23%), and 9q (q34-qter, 23%). We conclude that DNA copy number changes are frequent in follicular, medullary, and anaplastic thyroid carcinoma but rare in papillary carcinoma when studied by CGH, Loss of chromosome 22 is particularly common in follicular carcinoma, and it is associated with the widely invasive type.