Acidithiobacillus ferrivorans, sp nov.; facultatively anaerobic, psychrotolerant iron-, and sulfur-oxidizing acidophiles isolated from metal mine-impacted environments
Acidithiobacillus ferrivorans, sp nov.; facultatively anaerobic, psychrotolerant iron-, and sulfur-oxidizing acidophiles isolated from metal mine-impacted environments
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DOI：
10.1007/s00792-009-0282-y
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发表时间：
2010-01-01
期刊：
影响因子：
2.9
通讯作者：
Johnson, D. Barrie
中科院分区：
文献类型：
--
作者：
Hallberg, Kevin B.;Gonzalez-Toril, Elena;Johnson, D. Barrie
表型和基因型分析进行了四个铁和硫氧化嗜酸细菌（“NO-37组”）从世界不同地区分离。16 SrRNA同源性分析表明，它们之间有很高的亲缘关系，但与嗜酸氧化亚铁硫杆菌模式菌株的亲缘关系较低。NO-37群菌株是专性化能无机自养菌、兼性厌氧菌、重氮营养菌和耐冷菌。它们对极低pH的耐受性较差，与At相反。NO-37群菌株均为运动型。NO-37群菌株基因组DNA GC含量约为56mol%，与At.氧化亚铁（T）为37%。还似乎NO-37组的细菌具有与At不同的氧化亚铁的生化机制。氧化亚铁（T）;在NO-37组分离物的任何一个中均未检测到编码原型质朴花青苷（RusA）的基因，而是从四个新分离物中的三个中扩增出编码同源蛋白（RusB）的基因。NO-37组的分离物显然属于与已在嗜酸硫杆菌属中识别的那些不同的种，提议将嗜酸硫杆菌命名为嗜铁硫杆菌（Acidithiobacillus ferrivorans）。
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of Acidithiobacillus ferrooxidans. The NO-37 group isolates are obligate chemolithoautotrophs, facultative anaerobes, diazotrophic, and psychrotolerant. They are less tolerant of extremely low pH, and in contrast to At. ferrooxidans (T), all of the NO-37 group isolates are motile. The GC contents of genomic DNA of the NO-37 group isolates were around 56 mol% and the DNA-DNA hybridization value between genomic DNA of isolate NO-37 and At. ferrooxidans (T) was 37%. It also appears that the bacteria of the NO-37 group have a different biochemical mechanism for oxidizing ferrous iron than At. ferrooxidans (T); the gene coding for the archetypal rusticyanin (RusA) was not detected in any of the NO-37 group isolates, rather a gene coding for a homologous protein (RusB) was amplified from three of the four novel isolates. Isolates of the NO-37 group clearly belong to a species that is different to those already recognized in the genus Acidithiobacillus, for which the name Acidithiobacillus ferrivorans is proposed.