AXL, A TRANSFORMING GENE ISOLATED FROM PRIMARY HUMAN MYELOID-LEUKEMIA CELLS, ENCODES A NOVEL RECEPTOR TYROSINE KINASE
AXL, A TRANSFORMING GENE ISOLATED FROM PRIMARY HUMAN MYELOID-LEUKEMIA CELLS, ENCODES A NOVEL RECEPTOR TYROSINE KINASE
复制标题
DOI：
10.1128/mcb.11.10.5016
复制
发表时间：
1991-10-01
影响因子：
5.3
通讯作者：
LIU, ET
中科院分区：
文献类型：
--
作者：
OBRYAN, JP;FRYE, RA;LIU, ET
利用敏感的转染-致瘤性试验，我们从两个慢性粒细胞白血病患者的DNA中分离到一个新的转化基因。序列分析表明，该基因的产物axl是一种受体酪氨酸激酶。在NIH 3 T3细胞中过表达axl cDNA诱导肿瘤转化，伴随着140-kDa axl酪氨酸磷酸化蛋白的出现。axl cDNA在杆状病毒系统中的表达导致被抗磷酸酪氨酸抗体识别的适当重组蛋白的表达，从而证实axl蛋白是酪氨酸激酶。纤连蛋白III型和免疫球蛋白样重复序列的胞外结构域，以及不同的氨基酸序列的激酶结构域中的并列，表明axl蛋白代表一种新的受体酪氨酸激酶亚类。
Using a sensitive transfection-tumorigenicity assay, we have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the product of this gene, axl, is a receptor tyrosine kinase. Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic transformation with the concomitant appearance of a 140-kDa axl tyrosine-phosphorylated protein. Expression of axl cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by antiphosphotyrosine antibodies, confirming that the axl protein is a tyrosine kinase. The juxtaposition of fibronectin type III and immunoglobulinlike repeats in the extracellular domain, as well as distinct amino acid sequences in the kinase domain, indicate that the axl protein represents a novel subclass of receptor tyrosine kinases.