Human cytomegalovirus DNA: restriction enzyme cleavage maps and map locations for immediate-early, early, and late RNAs.
Human cytomegalovirus DNA: restriction enzyme cleavage maps and map locations for immediate-early, early, and late RNAs.
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DOI：
10.1016/0042-6822(81)90249-x
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发表时间：
1981-10
期刊：
影响因子：
3.7
通讯作者：
J. Demarchi
中科院分区：
文献类型：
--
作者：
J. Demarchi
人类巨细胞病毒（戴维斯株）DNA 的完整物理图谱是由限制性内切酶 HindIII、XbaI 和 EcoRI 产生的片段构建的。结果表明，人类巨细胞病毒基因组的结构组织与单纯疱疹病毒基因组相似：长（L）片段，占基因组的82％，与短（S）片段相连，占基因组的其余18％。 S 和 L 片段的排列产生四种不同的分子排列，它们在病毒粒子 DNA 制剂中以等摩尔比例表示。在映射于 S 片段的一个末端的片段内以及映射于 L 片段的一个末端的片段中检测到 DNA 大小的异质性。确定了感染后不同时间合成RNA的基因组区域。立即早期RNA（在放线菌酮存在下合成的RNA）仅限于基因组上的几个区域，但与映射在0.686和0.733单位之间（L段内）的区域大量杂交。早期的 RNA（在没有病毒 DNA 合成的情况下合成）与基因组的大部分区域杂交，但特别丰富的是映射在 0 到 0.046 个单位之间的小区域（也在 L 片段内）。在感染后期积累的RNA在基因组的任何区域都没有特别丰富的特征。
Complete physical maps for human cytomegalovirus (Davis strain) DNA were constructed from fragments produced by restriction endonucleasesHindIII,XbaI, andEcoRI. The results showed that the human cytomegalovirus genome has a structural organization similar to that of the herpes simplex virus genome: a long (L) segment, comprising 82% of the genome, joined to a short (S) segment, comprising the remaining 18% of the genome. Permutations of the S and L segments produce four different molecular arrangements which are represented in equimolar proportions in virion DNA preparations. Heterogeneity in the size of the DNA was detected within fragments which map at one terminus of the S segment and also in fragments which map at one terminus of the L segment. The regions of the genome from which RNA synthesized at various times after infection originates was determined. Immediate-early RNA (RNA synthesized in the presence of cycloheximide) was restricted to a few regions on the genome but hybridized in abundance to a region which mapped between 0.686 and 0.733 units (within the L segment). Early RNA (synthesized in the absence of viral DNA synthesis) hybridized to most areas of the genome, but in particular abundance to a small region which mapped between 0 and 0.046 units (also within the L segment). RNA which accumulated during the late phase of infection was not characterized by particular abundances to any region of the genome.