Measurement of contractile forces generated by individual fibroblasts on self-standing fiber scaffolds
Measurement of contractile forces generated by individual fibroblasts on self-standing fiber scaffolds
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DOI：
10.1007/s10544-010-9475-5
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Grigoropoulos, Costas P.
中科院分区：
文献类型：
--
作者：
Jeon, Hojeong;Kim, Eunpa;Grigoropoulos, Costas P.
伤口部位细胞的收缩性对了解病理性伤口愈合和制定治疗策略具有重要意义。特别是细胞产生的收缩力是设计人工三维细胞培养支架的基本要素。直接评估三维结构材料的变形已被用于计算收缩力平均总力相对于细胞种群数。然而，由于实验假设和细胞空间和时间反应的巨大差异，宏观方法只提供了收缩性的下限。在本研究中，为了测量单个细胞在通过飞秒激光诱导双光子聚合制备的自立纤维支架上产生的收缩力，在显微镜下检查了细胞收缩力。采用柱状屈曲模型对单个站立的纤维支架进行屈曲，可以减少以往宏观和微观收缩力测量研究中出现的实验假设和计算误差。通过量化不同直径纤维屈曲的偏心临界载荷，计算了30-116 nN范围内的单孔收缩力。在目前的研究中，一个力的大小约200毫安被认为是上界的收缩力施加的单细胞。此外，在241-709 nN的范围内，计算了单个纤维上多个细胞的收缩力。
Contractility of cells in wound site is important to understand pathological wound healing and develop therapeutic strategies. In particular, contractile force generated by cells is a basic element for designing artificial three-dimensional cell culture scaffolds. Direct assessment of deformation of three-dimensional structured materials has been used to calculate contractile forces by averaging total forces with respect to the cell population number. However, macroscopic methods have offered only lower bounds of contractility due to experimental assumptions and the large variance of the spatial and temporal cell response. In the present study, cell contractility was examined microscopically in order to measure contractile forces generated by individual cells on self-standing fiber scaffolds that were fabricated via femtosecond laser-induced two-photon polymerization. Experimental assumptions and calculation errors that arose in previous studies of macroscopic and microscopic contractile force measurements could be reduced by adopting a columnar buckling model on individual, standing fiber scaffolds. Via quantifying eccentric critical loads for the buckling of fibers with various diameters, contractile forces of single cells were calculated in the range between 30-116 nN. In the present study, a force magnitude of approximately 200 nN is suggested as upper bound of the contractile force exerted by single cells. In addition, contractile forces by multiple cells on a single fiber were calculated in the range between 241-709 nN.