THE SALMONELLA-WIEN VIRULENCE PLASMID PZM3 CARRIES TN1935, A MULTIRESISTANCE TRANSPOSON CONTAINING A COMPOSITE IS1936-KANAMYCIN RESISTANCE ELEMENT
THE SALMONELLA-WIEN VIRULENCE PLASMID PZM3 CARRIES TN1935, A MULTIRESISTANCE TRANSPOSON CONTAINING A COMPOSITE IS1936-KANAMYCIN RESISTANCE ELEMENT
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DOI：
10.1016/0147-619x(88)90028-5
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发表时间：
1988-11-01
期刊：
影响因子：
2.6
通讯作者：
CASALINO, M
中科院分区：
文献类型：
--
作者：
COLONNA, B;BERNARDINI, M;CASALINO, M
从文氏沙门氏菌IncFIme毒力质粒pZM3中分离到一个23.5kb的转座子Tn1935，该转座子介导对氨苄西林、卡那霉素、汞、壮观霉素和磺胺的抗性。Tn1935拥有Tn21的全部序列，并含有两个额外的DNA片段，分别为0.95kb和2.7kb，分别携带氨苄西林和卡那霉素抗性基因。后者是复合元件的一部分，因为它的两侧有两个直接定向的类似IS15的插入序列(IS1936)。IS1936全长约800bp，与IS15、IS26、IS46、IS140和IS176有密切的亲缘关系。对IS1936介导的协整合体的功能分析表明，两个插入序列都是活性的，并且能够以相同的频率形成协整合体。协整的解决需要主机REC系统的存在。Tn1935中复合IS1936元件的存在支持这样的假设，即多药耐药转座子是通过插入本身可转座的抗生素决定子而进化的。
Tn1935, a 23.5-kb transposon mediating resistance to ampicillin, kanamycin, mercury, spectinomycin, and sulfonamide was isolated from pZM3, an IncFIme virulence plasmid from Salmonella wien. Tn1935 possesses the entire sequence of Tn21 and contains two additional DNA segments of 0.95 and 2.7 kb carrying the ampicillin and kanamycin resistance genes, respectively. The latter is part of a composite element since it is flanked by two IS15-like insertion sequences (IS1936) in direct orientation. IS1936 is about 800 bp long and is closely related to IS15.DELTA., IS26, IS46, IS140, and IS176. Functional analysis of IS1936-mediated cointegrates shows that both insertion sequences are active and able to form cointegrates at the same frequency. Resolution of the cointegrates requires the presence of the host Rec system. The presence of the composite IS1936-element within Tn1935 supports the hypothesis that multidrug resistance transposons evolved by insertion of antibiotic determinants which are themselves transposable.