MiR-21 protected human glioblastoma U87MG cells from chemotherapeutic drug temozolomide induced apoptosis by decreasing Bax/Bcl-2 ratio and caspase-3 activity
MiR-21 protected human glioblastoma U87MG cells from chemotherapeutic drug temozolomide induced apoptosis by decreasing Bax/Bcl-2 ratio and caspase-3 activity
复制标题
MiR-21 通过降低 Bax/Bcl-2 比率和 caspase-3 活性，保护人胶质母细胞瘤 U87MG 细胞免受化疗药物替莫唑胺诱导的细胞凋亡
DOI：
10.1016/j.brainres.2010.07.009
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发表时间：
2010-09-17
期刊：
影响因子：
2.9
通讯作者：
Wang, Zhimin
中科院分区：
文献类型：
--
作者：
Shi, Lei;Chen, Jian;Wang, Zhimin
MicroRNAs(MiRNAs)是一种非编码的小RNA分子，通过切割或抑制靶mRNAs的翻译来调节蛋白质的表达。在哺乳动物中，它们的功能主要是通过与靶mRNAs的3‘非编码区不完全互补来抑制靶mRNAs的转录本。最近研究发现一些miRNAs参与了胶质瘤的发育调控，尤其是一些上调的miRNAs，如microRNA-21(miR-21)，已被发现在培养的多形性胶质母细胞瘤细胞中起癌基因的作用。替莫唑胺(TMZ)是一种烷基化药物，是治疗胶质母细胞瘤的一种有前途的化疗药物。然而，抗性发展迅速，出现频率高。为了探讨耐药机制，我们发现miR-21对TMZ诱导人胶质母细胞瘤U87 MG细胞的凋亡具有保护作用。我们的研究表明，TMZ能显著促进U87 MG细胞的凋亡(P<0.05)。
MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate protein expression by cleaving or repressing the translation of target mRNAs. In mammal animals, their function mainly represses the target mRNAs transcripts via imperfectly complementary to the 3'UTR of target mRNAs. Several miRNAs have been recently reported to be involved in modulation of glioma development, especially some upregulated miRNAs, such as microRNA-21 (miR-21), which has been found to function as an oncogene in cultured glioblastoma multiforme cells. Temozolomide (TMZ), an alkylating agent, is a promising chemotherapeutic agent for treating glioblastoma. However, resistance develops quickly and with high frequency. To explore the mechanism of resistance, we found that miR-21 could protect human glioblastoma U87MG cells from TMZ induced apoptosis. Our studies showed that TMZ markedly enhanced apoptosis in U87MG cells compared with untreated cells (P