Histological assessment in grafts of highly purified beta-tricalcium phosphate (OSferion®) in human bones
Histological assessment in grafts of highly purified beta-tricalcium phosphate (OSferion®) in human bones
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DOI：
10.1016/j.biomaterials.2005.08.034
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Endo, N
中科院分区：
文献类型：
--
作者：
Ogose, A;Kondo, N;Endo, N
在动物实验中，市场上可获得的β-磷酸三钙(β-TCP，OSferion(R))具有突出的骨传导活性和生物可降解性。我们通过常规苏木精-伊红染色、银染、免疫组织化学和原位杂交等方法，对4例受累于人骨的移植物OSferion(R)的组织学特征进行了分析。OSferion(R)在移植14天后，可通过破骨细胞的早期生物吸收、大毛孔的血管侵入和成骨细胞在陶瓷表面附着。移植28天后，原有的骨与OSferion(R)之间有明显的骨形成和直接骨连接。植骨后72周未见活跃的成骨细胞衬里和破骨细胞样巨细胞的附着。银浸渍显示在OSferion(R)的可能微孔中存在胶原纤维。(C)2005爱思唯尔有限公司。保留所有权利。
Prominent osteoconductive activity and the biodegradable nature of commercially available beta-tricalcium phosphate (beta-TCP, OSferion (R)) have been documented in animal experiments. We analyzed four cases of involving grafted OSferion (R) in human bone with respect to histological features by routine hematoxylin and eosin staining, silver impregnation, immunohistochemistry and in situ hybridization. OSferion (R) affords early bioresorption by osteoclasts, vascular invasion of macropores and osteoblastic cell attachment on the surface on the ceramic surface 14 days after grafting. Prominent bone formation and direct bone connection between preexisting bone and OSferion (R) were evident 28 days after grafting. Nearly the entire TCP surface was covered by lamellar bone; additionally, active osteoblastic lining and attachment of the osteoclast-like giant cells were not observed 72 weeks after grafting. Silver impregnation revealed the presence of collagen fibrils within probable micropores of OSferion (R). (c) 2005 Elsevier Ltd. All rights reserved.