Elemental bio-imaging using laser ablation-triple quadrupole-ICP-MS
Elemental bio-imaging using laser ablation-triple quadrupole-ICP-MS
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DOI：
10.1039/c5ja00293a
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发表时间：
2016-01-01
影响因子：
3.4
通讯作者：
Doble, Philip A.
中科院分区：
文献类型：
--
作者：
Bishop, David P.;Clases, David;Doble, Philip A.
组织切片中痕量金属分布的元素生物成像（EBI）通常通过激光烧蚀-单四极杆-电感耦合等离子体质谱（LA-ICP-SQ-MS）来执行，其中生物相关元素（例如Fe）的灵敏度可被多原子干扰抑制。三重四极杆ICP-MS（ICP-QQQ-MS）旨在减少干扰，并检查其在EBI中的潜在应用。三个调谐模式;无气体，H-2，和O-2/H-2调谐开发和研究其适用性。这三种方法都表现良好，具有高信噪比和低检测限。将O-2/H-2调谐应用于前列腺癌活检样品，在与癌症相关的区域中发现高水平的Zn。应用氧质量位移对小鼠脑中的P和Se进行成像。ICP-QQQ-MS独特的聚焦特性和使用混合池气体的能力为所有测量的同位素提供了灵敏的分析，其中包括O-2/H-2调谐，包括那些不受质量转移反应影响的同位素。
Elemental bio-imaging (EBI) of trace metal distributions in tissue sections is typically performed by laser ablation-single quadrupole-inductively coupled plasma-mass spectrometry (LA-ICP-SQ-MS) in which the sensitivity of biologically relevant elements such as Fe may be inhibited by polyatomic interferences. The triple quadrupole ICP-MS (ICP-QQQ-MS) is designed to reduce interferences and was examined for its potential application to EBI. Three tune modes; a no-gas, an H-2, and an O-2/H-2 tune were developed and investigated for their suitability. All three performed well with high signal to noise ratios and low limits of detection. The O-2/H-2 tune was applied to a prostate cancer biopsy sample, with high levels of Zn found in the area correlating with the cancer. The oxygen mass shift was applied to image P and Se in a mouse brain. The unique focussing properties of the ICP-QQQ-MS and the ability to use mixed cell gases provided sensitive analyses of all measured isotopes with the O-2/H-2 tune, including those unaffected by mass shift reactions.