A HISTIOCYTE-SPECIFIC MARKER IN THE DIAGNOSIS OF MALIGNANT FIBROUS HISTIOCYTOMA - USE OF MONOCLONAL-ANTIBODY KP-1 (CD68)
A HISTIOCYTE-SPECIFIC MARKER IN THE DIAGNOSIS OF MALIGNANT FIBROUS HISTIOCYTOMA - USE OF MONOCLONAL-ANTIBODY KP-1 (CD68)
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DOI：
10.1093/ajcp/97.6.759
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发表时间：
1992-06-01
影响因子：
3.5
通讯作者：
PINKUS, GS
中科院分区：
文献类型：
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作者：
BINDER, SW;SAID, JW;PINKUS, GS
KP-1 （CD68）是最近发现的针对存在于组织细胞和巨噬细胞上的细胞质表位的单克隆抗体。为了确定该标志物在恶性纤维组织细胞瘤（MFH）病例评估中的特异性和敏感性，我们使用该试剂和一组商业抗体对25例MFH和25例其他肿瘤（包括各种软组织肉瘤）的福尔马林固定石蜡切片进行染色。25例MFH中有18例（72%）检测到KP-1，而其他肿瘤均为阴性，包括12例MFH以外的多形性软组织肉瘤。KP-1染色的肿瘤细胞百分比各不相同。在11例病例中，KP-1仅局部存在，但染色强度高，并伴有少量非特异性或背景染色。来自故事状肿瘤的多形性组织细胞和梭形细胞在大多数情况下具有强烈的KP-1抗体，并且在肿瘤巨细胞上也存在抗原。尽管α -1抗胰蛋白酶和α -1凝乳胰蛋白酶在MFH病例中染色的比例较高（92%），但在其他肿瘤中也发现了这些标志物的免疫反应性。由于其作为组织细胞标志物的特异性，KP-1是一组抗体中有用的成分，用于软组织肉瘤的表征和MFH的诊断。
KP-1 (CD68) is a recently described monoclonal antibody to a cytoplasmic epitope present on tissue histiocytes and macrophages. To determine the specificity and sensitivity of this marker in the evaluation of cases of malignant fibrous histiocytoma (MFH), this reagent and a panel of commercially antibodies were used to stain formalin-fixed paraffin sections from 25 cases of MFH and 25 other tumors, including a variety of soft-tissue sarcomas. Eighteen of 25 cases of MFH stained for KP-1 (72%), whereas all other tumors were negative, including 12 cases of pleomorphic soft-tissue sarcoma other than MFH. The percentage of tumor cells staining for KP-1 varied. In 11 cases KP-1 was only focally present, but staining was of a high intensity and associated with minimal nonspecific or background staining. Pleomorphic histiocytic cells and spindle cells from storiform tumors were strongly decorated with antibodies to KP-1 in most cases, and antigen also was present on tumor giant cells. Although alpha-1-antitrypsin and alpha-1-chymotrypsin stained a higher percentage of cases of MFH (92%), immunoreactivity for these markers also was noted in other tumors. Because of its specificity as a histiocyte marker, KP-1 is a useful component in a panel of antibodies for the characterization of soft-tissue sarcomas and the diagnosis of MFH.