Nucleotide sequences of mRNAs encoding Epstein-Barr virus nuclear proteins: a probable transcriptional initiation site.
Nucleotide sequences of mRNAs encoding Epstein-Barr virus nuclear proteins: a probable transcriptional initiation site.
复制标题
编码 Epstein-Barr 病毒核蛋白的 mRNA 的核苷酸序列：可能的转录起始位点。
DOI：
10.1073/pnas.83.14.5096
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Kieff,E
中科院分区：
文献类型：
--
作者：
Sample,J;Hummel,M;Braun,D;Birkenbach,M;Kieff,E
分析了3个EB病毒第二核抗原(EBNA2)基因和2个EBNA1基因的cDNA克隆。两个EBNA2克隆从一个启动子的42个碱基3‘端开始，该启动子位于EB病毒的长内部重复序列中，很可能是EBNA2的启动子。令人惊讶的是，第一个拼接在两个不重叠的开放阅读框架的第一个开始处创建了一个Aug。第二个开放阅读框编码EBNA2。两个不完整的EBNA1mRNAc DNA克隆从EBNA2的两个外显子的一部分开始，并包含另外两个外显子，它们分别将19和59kb碱基3‘映射到EBNA2编码区。该基因的3‘外显子编码EBNA1。提出了一个调控这些RNA转录的模型。
Three cDNA clones of the second Epstein-Barr virus nuclear antigen (EBNA2) mRNA and two of the EBNA1 mRNA were analyzed. Two EBNA2 clones begin 42 bases 3' to a promoter in the Epstein-Barr virus long internal repeat, which is likely to be the EBNA2 promoter. Surprisingly, the first splice creates an AUG at the beginning of the first of two nonoverlapping open reading frames. The second open reading frame encodes EBNA2. Two incomplete EBNA1 mRNA cDNA clones begin with parts of two of the EBNA2 exons and contain two other exons that map 19 and 59 kilobases 3' to the EBNA2 coding domain. The 3' exon of this mRNA encodes EBNA1. A model for regulation of transcription of these RNAs is presented.