cDNA sequence analysis of seven peptide toxins from the spider Selenocosmia huwena
cDNA sequence analysis of seven peptide toxins from the spider Selenocosmia huwena
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DOI：
10.1016/j.toxicon.2003.08.007
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发表时间：
2003-12-01
期刊：
影响因子：
2.8
通讯作者：
Liang, SP
中科院分区：
文献类型：
--
作者：
Diao, JB;Lin, Y;Liang, SP
从虎纹捕鸟蛛（Selenocommiahuwena）中克隆了7个编码6种毒素HWTX-I、HWTX-II、HWTX-IIIa、HWTX-IV、HWTX-V、HWTX-VII和1种凝集素SHL-I的cDNA序列。根据它们的氨基酸序列，设计并合成了3 ′ RACE和5 ′ RACE引物。通过3'和5' RACE获得的两个部分cDNA序列的重叠，获得了它们的全长cDNA序列。这7个肽的cDNA都编码一个前体，包括一个21-24个残基的潜在信号肽，一个约30个残基的成熟毒素和一个插入的前区。HWTX-I、HWTX-IIIa、HWTX-IV、HWTX-V和SHL-I的前原区cDNA序列比较表明，它们的前原区具有高度的相似性，这与HWTX-I、HWTX-IV和SHL-I的抑制剂胱氨酸结相似，但它们的功能不同。HWTX-II和HWTX-VII具有与HWTX-I、HWTX-IV和SHL-I不同的高度相似的前原区。已确定HWTX-II的三维结构表现出不同的基序。这表明来自S.根据cDNA序列的前原区，虎纹虫可分为两个不同的超科。(C)2003 Elsevier Ltd.保留所有权利。
Seven cDNAs encoding six toxins HWTX-I, HWTX-II, HWTX-IIIa, HWTX-IV, HWTX-V, HWTX-VII and one lectin SHL-I, from the spider Selenocosmia huwena, were cloned and sequenced. On the basis of their amino acid sequences, we designed and synthesized 3' RACE and 5' RACE primer. By overlapping the two partial cDNA sequences obtained by 3' and 5' RACE, their full-length cDNA sequences were obtained. All of the cDNAs of these seven peptides encode a precursor including a potential signal peptide of 21-24 residues, a mature toxin of about 30 residues and an intervening pro region. The prepro regions of HWTX-I, HWTX-IIIa, HWTX-IV, HWTX-V and SHL-I were demonstrated, by the comparison of the cDNA sequences, to have high similarity, which is concert with the similar inhibitor cystine knot motif of HWTX-I, HWTX-IV and SHL-I although their functions are different. It was also demonstrated that, HWTX-II and HWTX-VII share the highly similar prepro region which is different from that of HWTX-I, HWTX-IV and SHL-I. The three dimensional structure of HWTX-II has been determined to exhibit a different motif. This indicates that the seven peptides from S. huwena could be classified into two different superfamilies according to the prepro region of cDNA sequences. (C) 2003 Elsevier Ltd. All rights reserved.