Dynamic fluorescence microscopy as a feasible technique for estimating the diffusion coefficients of small particles in the presence of additives.
Dynamic fluorescence microscopy as a feasible technique for estimating the diffusion coefficients of small particles in the presence of additives.
复制标题
动态荧光显微镜作为一种可行的技术，用于估计存在添加剂的小颗粒的扩散系数。
DOI：
10.1016/j.jcis.2003.06.002
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发表时间：
2003
影响因子：
9.9
通讯作者：
J. van Stam
中科院分区：
文献类型：
--
作者：
G. Carlsson;P. Warszyński;J. van Stam
用动态荧光显微镜研究了低浓度羧化聚苯乙烯胶乳在水溶液中的布朗运动。对于所有三种乳胶半径，即，0.5，0.265和0.1 μm时，如果考虑热效应和静电效应的影响，计算得到的扩散系数与理论预测吻合较好。它也是可能的区别胶乳与添加的聚合物的相互作用。添加的聚乙二醇显示没有或非常弱的相互作用与胶乳，直到聚合物重叠浓度达到，在该浓度下，聚合物网络的形成减缓了胶乳扩散。另一方面，聚乙烯吡咯烷酮与聚苯乙烯胶乳具有更显著的相互作用，并且即使在ppm范围内的聚合物浓度下也减缓了扩散。总的结论是，荧光显微镜是一种可行的方法来研究小颗粒在溶液中的动态行为。
The Brownian motion of carboxylated polystyrene latex, labeled with fluorescent probe molecules, at low concentrations in aqueous solutions was investigated by dynamic fluorescence microscopy. For all three latex radii, i.e., 0.5, 0.265, and 0.1 μm, the estimated diffusion coefficients correspond well with the theoretical predictions if thermal and electrostatic contributions are included in the discussions. It was also possible to discriminate latex interactions with an added polymer. Added polyethylene glycol showed no or very weak interaction with the latex until the polymer overlap concentration was reached, at which the formation of a polymer network slowed down the latex diffusion. Polyvinylpyrrolidone, on the other hand, had a more pronounced interaction with the polystyrene latex and slowed down the diffusion even at polymer concentrations in the ppm range. The overall conclusion is that fluorescence microscopy is a feasible method for the study of the dynamic behavior of small particles in solution.