Impact of a simulated gravity load for atmospheric reentry, 10 g for 2 min, on conscious mice
Impact of a simulated gravity load for atmospheric reentry, 10 g for 2 min, on conscious mice
复制标题
DOI：
10.1007/s12576-017-0526-z
复制
发表时间：
2017-07-01
影响因子：
2.3
通讯作者：
Takahashi, Satoru
中科院分区：
文献类型：
--
作者：
Morita, Hironobu;Yamaguchi, Aoi;Takahashi, Satoru
日本宇宙航空研究开发机构最近在国际空间站进行了一项小鼠实验，其中小鼠被饲养了35天，使用龙飞船取回，然后在溅落后2天收获用于分析。然而，回收程序的影响，即在大气层再入和溅落过程中将小鼠暴露于5-10 g 2分钟，是未知的。因此，本研究的目的是检查10 g负荷2 min（使用岐阜大学安装的具有1.5 m臂的贡多拉型离心机）对清醒小鼠的影响。血浆皮质酮在负荷后30 min增加，90 min恢复。在前庭核（VeN），下丘脑室旁核（PVN），杏仁核（CeA）的中央核中观察到显著的Fos表达。饲养行为和食物摄入受到抑制。前庭病变的小鼠表现出增加皮质酮和Fos在PVN的表达，但既不抑制食物摄入和饲养行为，也不增加Fos在VeN和CeA的表达。这些结果表明，模拟重力负荷诱导了短暂的应激反应，活动减退，和前庭介导的抑制食物摄入。
The Japan Aerospace Exploration Agency recently performed a mouse experiment in the International Space Station in which mice were raised for 35 days, retrieved using the Dragon spacecraft, and then harvested for analysis 2 days after splashdown. However, the impact of the retrieval procedure, which exposed mice to 5-10 g for 2 min during atmospheric reentry and splashdown, was unknown. Therefore, the purpose of this study was to examine the impact of a 10 g load for 2 min (using a gondola-type centrifuge with a 1.5-m arm installed at Gifu University) on conscious mice. Plasma corticosterone increased at 30 min after load application and recovered at 90 min. Significant Fos expression was observed in the vestibular nuclei (VeN), paraventricular hypothalamic nucleus (PVN), and central nucleus of the amygdala (CeA). Rearing behavior and food intake were suppressed. Mice with vestibular lesions demonstrated increased corticosterone and Fos expression in the PVN, but neither suppression of food intake and rearing behavior nor increased Fos expression in the VeN and CeA. These results suggest that the simulated gravity load induced a transient stress response, hypoactivity, and a vestibular-mediated suppression of food intake.