Terminal Maturation of Megakaryocytes and Platelet Production by Hematopoietic Stem Cells Irradiated with Heavy-Ion Beams
Terminal Maturation of Megakaryocytes and Platelet Production by Hematopoietic Stem Cells Irradiated with Heavy-Ion Beams
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DOI：
10.1667/rr2392.1
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发表时间：
2011-07-01
影响因子：
3.4
通讯作者：
Kashiwakura, Ikuo
中科院分区：
文献类型：
--
作者：
Monzen, Satoru;Takahashi, Kenji;Kashiwakura, Ikuo
造血术。过程，特别是巨核细胞生成和血小板生成，对高线性能量转移(LET)辐射高度敏感，例如重离子束，具有比低LET辐射更大的生物效应。这项研究检测了重离子束照射后巨核细胞的终末成熟和来自造血干细胞的血小板生成。将人胎盘/脐带血CD34(+)细胞暴露于单能碳离子束(LET=50keV/µm)，然后在添加有血小板生成素和白介素3的无血清培养液中进行培养。巨核细胞特异性标志物在未照射的对照细胞和照射的细胞中没有显著差异。照射后7d，早期造血受体Tie-2的表达显著增加，是对照细胞的1.31倍，Tie-2mRNA表达显著增加。此外，在重离子束照射的细胞中，PECAM1、SELP和CD44等mRNAs的表达也显著增加。而照射后细胞来源的血小板的黏附功能与对照组相比无明显差异。这些结果表明，尽管重离子束影响细胞黏附相关基因的表达，但重离子束照射后的造血干/祖细胞的巨核细胞生成和血小板生成的功能与未照射的细胞相似。(C)2011年，由辐射研究学会提供
Hematopoietic. processes, especially megakaryocytopoiesis and thrombopoiesis, are highly sensitive to high-linear energy transfer (LET) radiations such as heavy-ion beams that have greater biological effects than low-LET radiation. This study examined the terminal maturation of megakaryocytes and platelet production derived from hematopoietic stem cells irradiated with heavy-ion beams. CD34(+) cells derived from human placental/umbilical cord blood were exposed to monoenergetic carbon-ion beams (LET = 50 keV/mu m) and then cultured in a serum-free medium supplemented with thrombopoietin and interleukin-3. There was no significant difference in megakaryocyte-specific markers between nonirradiated control and irradiated cells. Expression of Tie-2, a receptor that acts in early hematopoiesis, showed a significant 1.31-fold increase after 2 Gy irradiation compared to control cells on day 7. There was a significant increase in Tie-2 mRNA expression. In addition, the expression of other mRNAs, such as PECAM1, SELP and CD44, was also significantly increased in cells irradiated with heavy-ion beams. However, the adherent function of platelets derived from the irradiated cells showed no difference from that in the controls. These results clarify that the functions of megakaryocytopoiesis and thrombopoiesis derived from hematopoietic stem/progenitor cells irradiated with heavy-ion beams are similar to those in the unirradiated cells, although heavy-ion beams affect the expression of genes associated with cellular adhesion. (C) 2011 by Radiation Research Society