Metabolomic comparison between natural Huaier and artificial cultured Huaier
Metabolomic comparison between natural Huaier and artificial cultured Huaier
复制标题
天然槐耳与人工培养槐耳的代谢组比较
DOI：
10.1002/bmc.5355
复制
发表时间：
2022-02-25
影响因子：
1.8
通讯作者：
Ling, Jianya
中科院分区：
文献类型：
--
作者：
Jian, Tongtong;Zhang, Yu;Ling, Jianya
刺槐Vanderbylia robiniophila(Murrill)B.K.(Huaier)是寄生在国槐和刺槐L树干上的一种高等真菌子实体。怀耳作为一种具有1600多年历史的传统中药，以其优异的抗癌活性而受到广泛关注。采用液-质联用技术对天然怀耳和人工养殖怀耳的代谢组学差异进行了系统的研究。主成分分析和潜在结构判别分析的正交投影分析结果表明，养殖怀耳与天然怀耳有明显的分离和个体分离，表明养殖怀耳与天然怀耳的代谢组学上存在差异。进一步进行系统聚类分析，根据不同代谢物和样品的代谢相似性对其进行聚类。天然怀耳含有较高的氨基酸、生物碱和萜类化合物，是抗癌或营养补充剂的极佳选择。Bel-7、402和A549细胞毒性试验结果表明，天然怀耳的抗癌活性优于人工怀耳。这可能是由于化学成分的不同，使得天然和养殖的怀耳具有不同的抗癌活性。
Vanderbylia robiniophila (Murrill) B.K. (Huaier) is a kind of higher fungal fruiting body that is parasitic on the trunk of Sophora japonica and Robinia pseudoacacia L.. As a traditional Chinese medicine with a history of more than 1,600 years, Huaier has attracted wide attention for its excellent anticancer activity. A systematic study on the metabolome differences between natural Huaier and artificial cultured Huaier was conducted using liquid chromatography-mass spectrometry in this study. Principal component analysis and orthogonal projection on latent structure-discriminant analysis results showed that cultured Huaier evidently separated and individually separated from natural Huaier, indicating metabolome differences between natural and cultured Huaier. Hierarchical clustering analysis was further performed to cluster the differential metabolites and samples based on their metabolic similarity. The higher contents of amino acids, alkaloids and terpenoids in natural Huaier make it an excellent choice as a traditional Chinese medicine for anticancer or nutritional supplementation. The results of the Bel-7,402 and A549 cell cytotoxicity tests showed that the anticancer activity of natural Huaier was better than that of cultured Huaier. This may be due to the difference in chemical composition, which makes the anticancer activities of natural and cultured Huaier different.