The plant-specific histone residue Phe41 is important for genome-wide H3.1 distribution.
The plant-specific histone residue Phe41 is important for genome-wide H3.1 distribution.
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DOI：
10.1038/s41467-018-02976-9
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发表时间：
2018-02-12
影响因子：
16.6
通讯作者：
Zhong X
中科院分区：
文献类型：
--
作者：
Lu L;Chen X;Qian S;Zhong X
组蛋白变体的动态掺入影响染色质结构和许多生物学过程。在拟南芥中，典型变体H3.1与H3.3在四个残基上不同，其中一个（H3.1Phe41）是植物中唯一且保守的。然而，它的进化意义仍然不清楚。在这里，我们发现Phe41首先出现在蕨类植物的H3.1中，并在陆地植物进化过程中变得稳定。与H3.1不同，H3.1F41Y变体在活跃转录区域获得异位积累。H3.1和H3.3之间的相互尾和核心结构域交换实验表明，H3.1核心虽然是必要的，但不足以将H3.1限制在沉默区域。我们的结论是维管植物特异性Phe41是H3.1基因组分布的关键，并可能与H3.1核心协同作用，以调节沉积模式。这项研究表明，Phe41可能已经进化到提供额外的调节植物中的组蛋白沉积。维管植物的典型组蛋白变体H3.1在位置41处含有植物界所特有的保守的Phe残基。在这里，Lu等人提供的证据表明H3.1Phe41与H3.1核心结构域协同作用，将H3.1沉积限制在基因组的沉默区域。
The dynamic incorporation of histone variants influences chromatin structure and many biological processes. In Arabidopsis, the canonical variant H3.1 differs from H3.3 in four residues, one of which (H3.1Phe41) is unique and conserved in plants. However, its evolutionary significance remains unclear. Here, we show that Phe41 first appeared in H3.1 in ferns and became stable during land plant evolution. Unlike H3.1, which is specifically enriched in silent regions, H3.1F41Y variants gain ectopic accumulation at actively transcribed regions. Reciprocal tail and core domain swap experiments between H3.1 and H3.3 show that the H3.1 core, while necessary, is insufficient to restrict H3.1 to silent regions. We conclude that the vascular-plant-specific Phe41 is critical for H3.1 genomic distribution and may act collaboratively with the H3.1 core to regulate deposition patterns. This study reveals that Phe41 may have evolved to provide additional regulation of histone deposition in plants. The canonical histone variant H3.1 of vascular plants contains a conserved Phe residue at position 41 that is unique to the plant kingdom. Here, Lu et al. provide evidence that H3.1Phe41 acts collaboratively with the H3.1 core domain to restrict H3.1 deposition to silent regions of the genome.
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影响因子：
10.5
作者：
Drane, Pascal;Ouararhni, Khalid;Hamiche, Ali
通讯作者：
Hamiche, Ali
H2A 去泛素酶 UBP12/13 是拟南芥多梳蛋白系统的一部分
影响因子：
18
作者：
Derkacheva, Maria;Liu, Shujing;Hennig, Lars
通讯作者：
Hennig, Lars
影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
通讯作者：
Allis CD
DOI：
10.1073/pnas.1635158100
发表时间：
2003-10-14
影响因子：
11.1
作者：
Hoek, M;Stillman, B
通讯作者：
Stillman, B
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF