Differential expression of alpha-E-catenin and alpha-N-catenin in the developing cerebral cortex
Differential expression of alpha-E-catenin and alpha-N-catenin in the developing cerebral cortex
复制标题
发育中的大脑皮层中α-E-连环蛋白和α-N-连环蛋白的差异表达
DOI：
10.1016/j.brainres.2005.12.057
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发表时间：
2006
期刊：
影响因子：
2.9
通讯作者：
A. Chenn
中科院分区：
文献类型：
--
作者：
A. Stocker;A. Chenn
Alpha (α) 连环蛋白可以调节细胞粘附和细胞增殖。在这里，我们描述了两种形式的 α-连环蛋白：αE-连环蛋白和 αN-连环蛋白在发育中的大脑皮层和初级皮层培养物中的表达。原位杂交和免疫荧光研究表明，αE-catenin在发育中的皮质脑室区的神经上皮前体细胞中高表达，而在皮质板中表达显着降低；相反，αN-连环蛋白在心室区表达较低，而在发育中的皮质板表达较高。在心室区，αE-连环蛋白和 αN-连环蛋白的免疫反应性在腔表面的环中富集，反映了与 β-连环蛋白一起在粘附连接处的定位。 αE-连环蛋白在原代皮质前体培养物中的表达最初很强，但随着神经前体分化成神经元而下降。 αN-连环蛋白在神经前体以及培养中分化的神经元中表达，反映了其在体内的表达模式。 αE-连环蛋白和 αN-连环蛋白的这些差异表达模式表明皮质发育过程中具有不同和重叠的功能。
Alpha (α) catenin proteins can regulate both cell adhesion and cell proliferation. Here, we characterize the expression of two forms of α-catenin, αE-catenin and αN-catenin, in the developing cerebral cortex and primary cortical cultures. In situ hybridization and immunofluorescence studies reveal that αE-catenin is highly expressed in neuroepithelial precursor cells in the developing cortical ventricular zone, with markedly reduced expression in the cortical plate; in contrast, αN-catenin expression is low in the ventricular zone and high in the developing cortical plate. In the ventricular zone, immunoreactivity for both αE-catenin and αN-catenin is enriched in rings at the lumenal surface, reflecting localization at adherens junctions together with β-catenin. Expression of αE-catenin in primary cortical precursor cultures is initially robust, but declines as neural precursors differentiate into neurons. Reflecting its expression pattern in vivo, αN-catenin is expressed in both neural precursors as well in neurons differentiated in culture. These differential expression patterns of αE-catenin and αN-catenin suggest both distinct and overlapping functions during cortical development.