High-throughput sequencing reveals the diversity of TCR β chain CDR3 repertoire in patients with severe acne
High-throughput sequencing reveals the diversity of TCR β chain CDR3 repertoire in patients with severe acne
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DOI：
10.1016/j.molimm.2020.01.024
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发表时间：
2020-04-01
影响因子：
3.6
通讯作者：
Wang, Jianqin
中科院分区：
文献类型：
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作者：
Shao, Lei;Liu, Yumei;Wang, Jianqin
痤疮是一种常见的慢性炎症性皮肤病，炎症免疫反应贯穿于痤疮皮损的各个阶段。在这项研究中，我们使用多重PCR和高通量测序技术相结合的方法来分析严重痤疮患者外周血中的T细胞受体β链CDR3(互补决定区3)。一旦与健康对照组比较，我们建议识别与痤疮相关的CDR3多肽。结果显示，严重寻常型痤疮(SA)患者外周血中T细胞受体β链(TRB)CDR3序列的多样性与正常对照组不同。此外，我们还发现10个Trb CDR3序列、氨基酸序列和V-J组合在SA组和非痤疮(NA)组之间的表达存在显著差异(P<；0.0001)。这些发现可能有助于更好地了解免疫在痤疮发病机制中的作用，并可能作为评估未来严重痤疮疾病风险或预后的生物标志物。
Acne is a common chronic inflammatory skin disease, and the inflammation immune response runs through all stages of acne lesions. In this study, we use a combination of multiplex-PCR and high-throughput sequencing technologies to analyze T cell receptor beta chain CDR3 (complementarity-determining region 3) in peripheral blood isolated from severe acne patients. Once compared with healthy controls, we propose to identify acne-relevant CDR3 peptides. Our results reveal that the diversity of T cell receptor beta chain (TRB) CDR3 sequences in the peripheral blood of the severe acne vulgaris (SA) group differed from that of the control group. In addition, we find 10 TRB CDR3 sequences, amino acid sequences and V-J combinations with significantly different expressions between the SA group and the non-acne (NA) group (P< 0.0001). These findings may contribute to a better understanding of the role of immunity in the pathogenesis of acne and may serve as biomarkers for evaluating risk or prognosis of severe acne disease in future.