Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses
Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses
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DOI：
10.3390/microorganisms8071014
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发表时间：
2020-07-01
期刊：
影响因子：
4.5
通讯作者：
Takahashi, Akira
中科院分区：
文献类型：
--
作者：
Kojima, Mizuki;Mawatari, Kazuaki;Takahashi, Akira
甲型流感病毒（iav）对人类及其牲畜构成严重的全球性威胁。本研究旨在确定紫外发光二极管（uv - led）对IAV消毒的理想照射。我们使用8个UV- led（峰值波长为365、310、300、290、280、270和260 nm）或一个低压汞灯（峰值波长为254 nm）对IAV H1N1亚型进行4.8 mJ/cm(2)紫外照射。通过对Madin-Darby犬肾（MDCK）细胞或鸡胚蛋的感染率进行失活评价。260 nm UV-LED的失活效果最好。由于辐照诱导的失活效应与病毒RNA的损伤密切相关，我们计算了辐照光谱与病毒RNA吸收之间的相关系数（R-AE）。TheR（AE）得分与UV-LEDs和LP-UV灯灭活密切相关。为了提高theR（AE）评分，我们将三种不同峰值的WL UV-LED（混合UV-LED）组合在一起。混合UV-LED （R-AE= 86.3）比260 nm （R-AE= 68.6）和270 nm (R-AE= 42.2) UV-LED对H1N1和H6N2亚型的灭活效果更显著。TheR（AE）评分是提高UV-LED辐照的抗病毒效果的重要因素。
Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock. This study aimed to determine the ideal irradiation by ultraviolet-light emitting diodes (UV-LEDs) for IAV disinfection. We irradiated the IAV H1N1 subtype with 4.8 mJ/cm(2)UV using eight UV-LEDs [peak wavelengths (WL) = 365, 310, 300, 290, 280, 270, and 260 nm)] or a mercury low pressure (LP)-UV lamp (Peak WL = 254 nm). Inactivation was evaluated by the infection ratio of Madin-Darby canine kidney (MDCK) cells or chicken embryonated eggs. Irradiation by the 260 nm UV-LED showed the highest inactivation among all treatments. Because the irradiation-induced inactivation effects strongly correlated with damage to viral RNA, we calculated the correlation coefficient (R-AE) between the irradiant spectrum and absorption of viral RNA. TheR(AE)scores strongly correlated with the inactivation by the UV-LEDs and LP-UV lamp. To increase theR(AE)score, we combined three different peak WL UV-LEDs (hybrid UV-LED). The hybrid UV-LED (R-AE= 86.3) significantly inactivated both H1N1 and H6N2 subtypes to a greater extent than 260 nm (R-AE= 68.6) or 270 nm (R-AE= 42.2) UV-LEDs. TheR(AE)score is an important factor for increasing the virucidal effects of UV-LED irradiation.