OVARIAN SURFACE EPITHELIUM - AUTONOMOUS PRODUCTION OF CONNECTIVE TISSUE-TYPE EXTRACELLULAR-MATRIX
OVARIAN SURFACE EPITHELIUM - AUTONOMOUS PRODUCTION OF CONNECTIVE TISSUE-TYPE EXTRACELLULAR-MATRIX
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DOI：
10.1095/biolreprod44.4.717
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发表时间：
1991-04-01
影响因子：
3.6
通讯作者：
KRUK, PA
中科院分区：
文献类型：
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作者：
AUERSPERG, N;MACLAREN, IA;KRUK, PA
已知卵巢表面上皮（OSE）通过在卵泡破裂部位的增殖和迁移以及通过基底膜的沉积来促进排卵后卵巢皮质的修复。 我们研究了其他细胞外基质成分的生产在培养的OSE细胞的大鼠（ROSE），使用免疫荧光显微镜，电子显微镜，和脯氨酸掺入。 我们比较了最近在低传代中培养的细胞，永生细胞系ROSE 239，其生长模式类似于低传代培养物，以及永生细胞系ROSE 199，其形成脊和乳头。 角蛋白和层粘连蛋白的存在证实了所有三种细胞类型的上皮性质。 所有三种细胞类型分泌I型和III型胶原蛋白，至少一种（ROSE 199）产生高度聚合的带状原纤维，这是基质或间质细胞外基质的特征。 同时生产的胶原蛋白I型和III型，角蛋白，和层粘连蛋白的克隆亚群排除了混合上皮/成纤维细胞群体的线的起源。 结果表明，OSE具有合成结缔组织基质主要成分的能力。 他们认为，这种上皮细胞，除了其假定的蛋白水解作用，也可能表达合成活性的卵巢皮质间质的重塑。 OSE细胞自主产生基质成分的能力可能是卵巢表面乳头形成和OSE衍生卵巢癌肿瘤进展的重要因素。
The ovarian surface epithelium (OSE) is known to contribute to postovulatory repair of the ovarian cortex by proliferation and migration over the site of follicular rupture, and by deposition of a basement membrane. We examined the production of other extracellular matrix components in culture by OSE cells of the rat (ROSE), using immunofluorescence microscopy, electron microscopy, and proline incorporation. We compared recently explanted cells in low passage, the immortal line ROSE 239, whose growth pattern resembles low passage cultures, and the immortal line ROSE 199, which forms ridges and papillae. The epithelial nature of all three cell types was confirmed by the presence of keratin and laminin. All three cell types secreted collagen types I and III and at least one (ROSE 199) produced highly polymerized banded fibrils, which are characteristic for stromal or interstitial extracellular matrix. Simultaneous production of collagen types I and III, keratin, and laminin by cloned subpopulations ruled out an origin of the lines in mixed epithelial/fibroblast populations. The results demonstrate that OSE has the capacity to synthesize major components of connective tissue stroma. They suggest that this epithelium, in addition to its postulated proteolytic role, may also express synthetic activity in the remodelling of the ovarian cortical stroma. A capacity of OSE cells to produce stromal components autonomously might be an important factor in the formation of ovarian surface papillae and in neoplastic progression of OSE-derived ovarian carcinomas.