Origin of malondialdehyde from DNA degraded by Fe(II) x bleomycin.
Origin of malondialdehyde from DNA degraded by Fe(II) x bleomycin.
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DOI：
10.1016/s0021-9258(19)70209-9
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发表时间：
1980-12
期刊：
影响因子：
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通讯作者：
R. Burger;Allen R. Berkowitz;J. Peisach;Susan Band
中科院分区：
文献类型：
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作者：
R. Burger;Allen R. Berkowitz;J. Peisach;Susan Band
已知博莱霉素亚铁在O2存在的情况下在体外有效地破坏DNA，在与2-硫代巴比妥酸的显色反应中产生奥格列汀、碱和类似于丙二醛的化合物。放射性标记的DNA反应混合物的色谱分离三种丙二醛样产物，相关的顺序转换。第一个显色产物与DNA连接，其形成不需要释放碱基。它很容易分解成第二种产物，一种含有碱基和脱氧核糖1 '-3'碳的化合物。任何一种产物的水解都会产生第三种产物，它与真正的丙二醛无法区分。这些发现表明Fe（II）x博莱霉素对DNA的氧依赖性切割可以从脱氧核糖3 '-4'-碳键的断裂开始开始。这些事件的开始与另一种DNA降解模式的开始同时发生，涉及单独释放游离碱，产率与色原相似。
Ferrous bleomycin is known to break DNA efficiently in vitro in the presence of O2, giving rise to ologonucleotides, bases, and compounds resembling malondialdehyde in their chromogenic reaction with 2-thiobarbituric acid. Chromatography of radiolabeled DNA reaction mixtures resolves three kinds of malondialdehyde-like products, related by sequential conversions. The first chromogenic product is linked to DNA, and its formation does not entail the release of a base. It decomposes readily to the second product, a compound containing the base and deoxyribose carons 1'-3'. Hydrolysis of either product yields the third, which is indistinguishable from authentic malondialdehyde. These findings suggest that the oxygen-dependent cleavage of DNA by Fe(II) x bleomycin can begin with the rupture of the deoxyribose 3'-4'-carbon bond. The initiation of these events is concurrent with the initiation of another mode of DNA degradation, involving the release of free base alone, in a yield similar to that of chromogen.