Three-Dimensional High-Resolution Rotational Tracking with Superlocalization Reveals Conformations of Surface-Bound Anisotropic Nanoparticles
Three-Dimensional High-Resolution Rotational Tracking with Superlocalization Reveals Conformations of Surface-Bound Anisotropic Nanoparticles
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DOI：
10.1021/nl304764w
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发表时间：
2013-03-01
期刊：
影响因子：
10.8
通讯作者：
Fang, Ning
中科院分区：
文献类型：
--
作者：
Marchuk, Kyle;Ha, Ji Won;Fang, Ning
直接追踪各向异性纳米粒子的三维旋转运动的能力将极大地增进我们对纳米粒子与表面相互作用方式的理解。在此，我们展示双色全内反射散射显微镜作为一种工具，用于探测等离子体金纳米棒与功能表面的相互作用。通过利用短轴和长轴表面等离子体共振散射增强，我们能够以同样高的分辨率解析金纳米棒相对于样品表面的面内和面外运动。结合通过点扩散函数拟合进行的超定位，我们克服了物镜焦平面内金纳米棒的四象限角度简并性，并以前所未有的细节解析了表面结合的各向异性纳米粒子的构象。
The ability to directly follow three-dimensional rotational movement of anisotropic nanoparticles will greatly enhance our understanding of the way nanoparticles interact with surfaces. Herein, we demonstrate dual-color total internal reflection scattering microscopy as a tool to probe the interactions of plasmonic gold nanorods with functional surfaces. By taking advantage of both the short and long axis surface plasmon resonance scattering enhancement, we are able to decipher both in-plane and out-of-plane gold nanorod motion relative to the sample surface with equally high resolution. In combination with superlocalization through point spread function fitting, we overcome the four-quadrant angular degeneracy of gold nanorods in the focal plane of the objective and resolve conformations of surface-bound anisotropic nanoparticles in unprecedented detail.