Preparation and characterization of cross-linked carboxymethyl chitin porous membrane scaffold for biomedical applications.
Preparation and characterization of cross-linked carboxymethyl chitin porous membrane scaffold for biomedical applications.
复制标题
DOI：
10.1016/j.carbpol.2015.02.050
复制
发表时间：
2015-08
影响因子：
11.2
通讯作者：
Liqing Zhao;Yiguang Wu;Shu Chen;Tao Xing
中科院分区：
文献类型：
--
作者：
Liqing Zhao;Yiguang Wu;Shu Chen;Tao Xing
采用溶胶-凝胶冷冻干燥法制备了多孔真皮支架膜。该方法首先将羧甲基甲壳素（CMC）水溶胶与1-乙基-3-[3-二甲氨基丙基]碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）交联，然后冻干，得到PDSM。本研究首次将交联CMC作为三维PDSM的唯一组分。研究了交联条件对PDSM性能的影响。当EDC：NHS：CMC质量比为5：3：10时，交联时间为4 h，CMC质量分数为4%时，PDSM的性能最佳。优化后的PDSM孔隙率大于90%，吸水膨胀率大于4000%。孔径分布均匀，在100 μm ~ 200 μm之间。拉伸强度在0.09MPa以上。在0.2mg/mL的溶菌酶溶液中，12天内，PDSM的降解率可达80%以上。最重要的是，PDSM没有细胞毒性和良好的生物相容性从MTT法。实验结果表明，PDSM可作为皮肤组织工程的真皮支架材料。
Porous dermal scaffold membrane (PDSM) was successfully prepared by using a so-called sol–gel freeze-drying method. In this method, the carboxymethyl chitin (CMC) hydrosol was first cross-linked by 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and then lyophilized to form the PDSM. For the first time, this research focused on the cross-linked CMC as the only component for three-dimensional PDSM. The effects of cross-linking conditions on the performance of the PDSM were investigated. And PDSM with optimal performance was obtained through 4-h cross-linking at 4 wt% of CMC concentration in the hydrosol, where the mass ratio of EDC to NHS to CMC was 5:3:10. The porosity of the optimized PDSM was more than 90% and the water swelling rate was above 4000%. The pore size was well distributed and was between 100 μm and 200 μm. And the tensile strength was above 0.09 MPa. The as-made PDSM could be degraded above 80% in 12 days in the presence of a 0.2 mg/mL lysozyme solution. Very importantly, the PDSM had no cytotoxicity and good biocompatibility from MTT assays. Our results showed the application possibility of the as-prepared PDSM as dermal scaffold for skin tissue engineering.