Functional relationship heavy metal P-type ATPases (OsHMA 2 and OsHMA3) of rice ( Oryza sativa ) using RNAi
Functional relationship heavy metal P-type ATPases (OsHMA 2 and OsHMA3) of rice ( Oryza sativa ) using RNAi
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DOI：
10.5511/plantbiotechnology.13.0616a
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发表时间：
2013-12
影响因子：
1.6
通讯作者：
Namiko Satoh-Nagasawa;Mikako Mori;K. Sakurai;Hidekazu Takahashi;A. Watanabe;H. Akagi
中科院分区：
文献类型：
--
作者：
Namiko Satoh-Nagasawa;Mikako Mori;K. Sakurai;Hidekazu Takahashi;A. Watanabe;H. Akagi
水稻重金属 P 型 ATP 酶 3 (OsHMA3) 在水稻品种‘Cho-Ko-Koku (CKK)’和‘Anjana Dhan’的镉 (Cd) 超积累中发挥着重要作用。另一方面，在芽中积累 Cd 水平非常低的突变体表明了一些基因的功能，例如 OsHMA2（这是将 Cd 加载到水稻中所必需的）。木质部）或水稻天然抗性相关巨噬细胞蛋白 5（OsNRAMP5，调节根部对镉从土壤的吸收）。尽管许多研究都考察了金属转运蛋白，但很少有人研究植物中多基因组合的功能。在此，我们首先使用RNA干扰(RNAi)方法下调OsHMA3并恢复OsHMA3表达被强烈抑制的植物。其次，OsHMA3 RNAi 植物与 oshma2 突变体的杂交揭示了植物中 OsHMA3 和 OsHMA2 之间的功能关系。我们得出的结论是，OsHMA2 功能的完全丧失对于最大限度地减少 Cd 从根到芽的转运至关重要。目前的研究结果还表明，在没有蛋白质 C 末端区域的情况下，OsHMA2 的活性可以在植物根部加速。
Oryza sativa heavy metal P-type ATPase 3 (OsHMA3) plays a major role in the hyperaccumulation of cadmium (Cd) by the rice cultivars ‘Cho-Ko-Koku (CKK)’ and ‘Anjana Dhan.’ On the other hand, mutants that accumulate Cd in the shoots at a very low level have suggested the function of several genes such as OsHMA2 (which is neccesary to load Cd into xylem) or O. sativa natural resistance-associated macrophage protein 5 (OsNRAMP5, which regulates the uptake of Cd from soil into the roots). Although many studies have examined metal transporters, few have investigated the function of combination of multiple gene in plant. Herein, first, we used an RNA interference (RNAi) method to down regulate OsHMA3 and recovered plants in which the expression of OsHMA3 was strongly suppressed. Second, crossing of OsHMA3 RNAi plants with oshma2 mutants revealed a functional relationship between OsHMA3 and OsHMA2 in plant. We concluded that a complete loss of function of OsHMA2 is crucial to minimize Cd translocation from the roots to the shoots. The current findings also implied that the activity of OsHMA2 can be accelerated in plant in the roots without the C-terminal region of the protein.