Hydantoin-hydrolysing enzymes for the enantioselective production of amino acids: new insights and applications
Hydantoin-hydrolysing enzymes for the enantioselective production of amino acids: new insights and applications
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DOI：
10.1016/j.tetasy.2004.07.061
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发表时间：
2004-09-20
影响因子：
--
通讯作者：
Dorrington, RA
中科院分区：
文献类型：
--
作者：
Burton, SG;Dorrington, RA
本报告介绍了海因酶生物催化领域的最新发现和新应用，特别关注立体选择性反应和立体控制的新机理理解以及新的对映体纯产品和新的应用。乙内酰脲水解系统包括乙内酰脲酶和N-氨基甲酰基氨基酸酰胺水解酶（NCAAH），它们依次分别催化乙内酰脲的水解开环和N-氨基甲酰基的水解裂解，产生对映体纯的氨基酸，其可以是非天然的，并且可以具有L-或D-构型。(C)2004爱思唯尔有限公司保留所有权利。
This report describes recent discoveries and new applications in the field of hydantoinase biocatalysis with particular attention to stereoselective reactions and new mechanistic understanding of stereocontrol as well as new enantiopure products and novel applications. Hydantoin-hydrolysing systems comprise hydantoinase and N-carbamoylamino acid amidohydrolase (NCAAH) enzymes, which sequentially catalyse hydrolytic hydantoin ring opening and hydrolytic cleavage of N-carbamoyl groups, respectively, yielding enantiopure amino acids, which may be non-natural, and may have the L- or D-configuration. (C) 2004 Elsevier Ltd. All rights reserved.