Structure of a ribosomal protein gene in Mucor racemosus.
Structure of a ribosomal protein gene in Mucor racemosus.
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总状毛霉核糖体蛋白基因的结构。
DOI：
10.1093/nar/17.22.9319
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发表时间：
1989
影响因子：
14.9
通讯作者：
Sypherd,PS
中科院分区：
文献类型：
--
作者：
Sosa,L;Fonzi,WA;Sypherd,PS
作为我们对毛霉总状体翻译装置分析的延伸，我们分离了一个编码毛霉核糖体蛋白的基因。基于为s开发的方法。通过杂交选择和体外翻译，我们鉴定了一个含有amucor核糖体蛋白基因组拷贝的λ-Charon 4A克隆。该基因由两个外显子组成，分别含有57和387个核苷酸。两个外显子被131个核苷酸的内含子隔开。经过处理的转录本全长714个核苷酸，包含一个25个核苷酸未转录的前导和一个114个核苷酸未转录的3 '端。该蛋白包含148个氨基酸，与非洲爪蟾S19核糖体蛋白同源性为61%。该基因的启动子区域含有与在inS中发现的theRPGandHomollpromoter元件高度同源的序列。酵母。Southern blot分析表明毛霉菌基因组包含该基因的三个拷贝。
As an extension of our analysis of the translational apparatus ofMucor racemosuswe have isolated a gene encoding a ribosomal protein ofMucor. Based on a method developed forS. cerevisiae, we identified by hybrid selection andin vitrotranslation a λ-Charon 4A clone containing the genomic copy of aMucorribosomal protein. The gene consisted of two exons of 57 and 387 nucleotides. The two exons were separated by an 131 nucleotide intron. The processed transcript was 714 nucleotides in length and contained a 25 nucleotide untranscribed leader and an 114 nucleotide untranscribed 3′-end. The protein predicted from the nucleotide sequence contained 148 amino acids and exhibited 61% identity with the S19 ribosomal protein ofXenopus laevis. The promoter region of the gene contained sequences highly homologous to theRPGandHomollpromoter elements found inS. cerevisiae. Southern blot analysis indicated that theMucor genomecontains three copies of this gene.