Emerging technologies for salivaomics in cancer detection.
Emerging technologies for salivaomics in cancer detection.
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DOI：
10.1111/jcmm.13007
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发表时间：
2017-04
影响因子：
5.3
通讯作者：
Wong DT
中科院分区：
文献类型：
--
作者：
Kaczor-Urbanowicz KE;Martín Carreras-Presas C;Kaczor T;Tu M;Wei F;Garcia-Godoy F;Wong DT
唾液诊断在许多癌症的早期发现和预防方面具有巨大的潜力。如果严格和有效地实施，它可以改善患者的生存时间并实现疾病的早期诊断。最近，人们在开发非侵入性技术方面做出了巨大的努力，这种技术可以在没有复杂和昂贵的程序的情况下应用。唾液是一种生物流体，已被证明具有优异的特性，可以用作诊断流体，因为除了血液或其他体液外，许多癌症的生物标志物也可以在整个唾液中找到。目前公认的生物标志物开发金标准方法包括色谱、质谱、凝胶电泳、微阵列和基于聚合酶链反应的定量。然而，随着与即时诊断、RNA测序、电化学检测和液体活检相关的新技术的快速发展，唾液诊断是一个蓬勃发展的领域。这些技术将有助于引入基于人群的筛查项目，从而实现早期检测、预后评估和疾病监测。这篇综述的目的是全面更新基于唾液的新兴诊断技术和早期检测癌症疾病的工具。
Salivary diagnostics has great potential to be used in the early detection and prevention of many cancerous diseases. If implemented with rigour and efficiency, it can result in improving patient survival times and achieving earlier diagnosis of disease. Recently, extraordinary efforts have been taken to develop non‐invasive technologies that can be applied without complicated and expensive procedures. Saliva is a biofluid that has demonstrated excellent properties and can be used as a diagnostic fluid, since many of the biomarkers suggested for cancers can also be found in whole saliva, apart from blood or other body fluids. The currently accepted gold standard methods for biomarker development include chromatography, mass spectometry, gel electrophoresis, microarrays and polymerase chain reaction‐based quantification. However, salivary diagnostics is a flourishing field with the rapid development of novel technologies associated with point‐of‐care diagnostics, RNA sequencing, electrochemical detection and liquid biopsy. Those technologies will help introduce population‐based screening programs, thus enabling early detection, prognosis assessment and disease monitoring. The purpose of this review is to give a comprehensive update on the emerging diagnostic technologies and tools for the early detection of cancerous diseases based on saliva.