A critical role of the KCa3.1 channel in mechanical stretch-induced proliferation of rat bone marrow-derived mesenchymal stem cells
A critical role of the KCa3.1 channel in mechanical stretch-induced proliferation of rat bone marrow-derived mesenchymal stem cells
复制标题
K Ca 3.1 通道在机械拉伸诱导大鼠骨髓衍生间充质干细胞增殖中的关键作用
DOI：
10.1111/jcmm.15014
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发表时间：
2020-02-17
影响因子：
5.3
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Jia, Xiaoling;Su, Hao;Fan, Yubo
机械刺激是调节间充质干细胞（MSC）增殖等功能的重要因素。Ca 2+激活的K+通道K（Ca）3.1在MSC增殖中起关键作用，但其在MSC增殖的机械调节中的作用仍然未知。在这里，我们研究了K（Ca）3.1通道的表达及其在大鼠骨髓来源的MSC（BMSC）增殖中的作用，以响应机械拉伸。应用机械牵张通过促进细胞周期进程刺激BMSC增殖。这种机械刺激上调K（Ca）3.1通道表达，并且K（Ca）3.1通道的药理学或遗传学抑制强烈抑制牵张诱导的细胞增殖和细胞周期进展的增加。这些结果支持K（Ca）3.1通道在机械力对MSC增殖的转导中起重要作用。我们的发现为机械刺激如何调节MSC增殖提供了新的机制见解，也为改善MSC增殖提供了可行的生物工程方法。
Mechanical stimulation is an important factor regulating mesenchymal stem cell (MSC) functions such as proliferation. The Ca2+-activated K+ channel, K(Ca)3.1, is critically engaged in MSC proliferation but its role in mechanical regulation of MSC proliferation remains unknown. Here, we examined the K(Ca)3.1 channel expression and its role in rat bone marrow-derived MSC (BMSC) proliferation in response to mechanical stretch. Application of mechanical stretch stimulated BMSC proliferation via promoting cell cycle progression. Such mechanical stimulation up-regulated the K(Ca)3.1 channel expression and pharmacological or genetic inhibition of the K(Ca)3.1 channel strongly suppressed stretch-induced increase in cell proliferation and cell cycle progression. These results support that the K(Ca)3.1 channel plays an important role in transducing mechanical forces to MSC proliferation. Our finding provides new mechanistic insights into how mechanical stimuli regulate MSC proliferation and also a viable bioengineering approach to improve MSC proliferation.