RAPID METHOD FOR ISOLATION OF FUNCTIONAL THYMUS-DERIVED MURINE LYMPHOCYTES
RAPID METHOD FOR ISOLATION OF FUNCTIONAL THYMUS-DERIVED MURINE LYMPHOCYTES
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DOI：
10.1002/eji.1830031011
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发表时间：
1973-01-01
影响因子：
5.4
通讯作者：
HERZENBERG, LA
中科院分区：
文献类型：
--
作者：
JULIUS, MH;SIMPSON, E;HERZENBERG, LA
描述了一种快速去除免疫球蛋白携带细胞的方法，该方法可有效地从预置或未预置的小鼠脾和淋巴结细胞悬液中去除免疫球蛋白。细胞悬浮液在尼龙羊毛色谱柱中37°C孵育45分钟后，免疫球蛋白阳性细胞减少9~100倍，T细胞增加1.5~2倍。与未分离的脾细胞相比，脾细胞通过这些色谱柱的流出细胞几乎不具有B前体细胞和记忆细胞活性，但包含了所有的辅助细胞和细胞毒效应细胞前体活性。
A rapid method is described for effectively removing immunoglobulin‐bearing cells from either primed or unprimed mouse spleen and lymph node cell suspensions. Incubation of cell suspensions in nylon wool columns for 45 min at 37 °C resulted in a 9 to 100‐fold depletion of immunoglobulin‐bearing cells and a complementary 1.5 to 2‐fold enrichment of T cells in the column effluent populations.The effluent population, derived from passage of spleen cells through these columns, was virtually devoid of B precursor and memory cell activity, but contained all of the helper cell and cytotoxic effector cell precursor activity when compared to unfractionated spleen cells.