The role of moderate static magnetic fields on biomineralization of osteoblasts on sulfonated polystyrene films
The role of moderate static magnetic fields on biomineralization of osteoblasts on sulfonated polystyrene films
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DOI：
10.1016/j.biomaterials.2011.06.053
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发表时间：
2011-11-01
期刊：
影响因子：
14
通讯作者：
Rafailovich, Miriam H.
中科院分区：
文献类型：
--
作者：
Ba, Xiaolan;Hadjiargyrou, Michael;Rafailovich, Miriam H.
我们研究了中等静磁场（SMF）对小鼠 MC3T3-E1 成骨细胞的影响，发现它们可以增强增殖并促进分化。在部分磺化聚苯乙烯（SPS，磺化度：33%）上生长的培养物中，响应 SMF 的增殖率的增加比在组织培养塑料上生长的培养物更大。我们之前已经证明，当磺化程度超过临界值（12%）时[1]，会发生自发纤维形成，这使得可以在外部场的影响下直接观察ECM纤维组织。我们发现，在存在 SMF 的情况下，在 SPS 上生长的培养物中产生的 ECM 组装成尺寸大于在不存在 SMF 的情况下生长的培养物的 ECM。在生物矿化过程的早期阶段（第 7 天），暴露于 SMF 的培养物也比对照培养物更快地模板化矿物质沉积。快速反应归因于血清中已经存在的抗磁性 ECM 蛋白的定向，然后可以启动进一步的细胞信号传导。 SMF 还影响晚期成骨细胞分化，通过 SFM 暴露 15 天后骨钙素分泌率和基因表达的增加来衡量。这与矿物质沉积和细胞模量的大幅增加相关。 GIXD 和 EDXS 分析证实了结晶羟基磷灰石的早期沉积。之前关于中等 SMF 影响的研究主要集中在细胞基因和蛋白质表达上，但没有考虑 ECM 纤维的组织。我们形成这些纤维的能力使我们能够探索这种附加效应，并强调其在生物矿化过程启动中的重要性。 (C) 2011 Elsevier Ltd. 保留所有权利。
We have investigated the effects of moderate static magnetic fields (SMFs) on murine MC3T3-E1 osteoblasts, and found that they enhance proliferations and promote differentiation. The increase in proliferation rates in response to SMFs was greater in cultures grown on partially sulfonated polytstyrene (SPS, degree of sulfonation: 33%) than in cultures grown on tissue culture plastic. We have previously shown that when the degree of sulfonation exceeded a critical value (12%) [1], spontaneous fibrillogenesis occured which allowed for direct observation of the ECM fibrillar organization under the influence of external fields. We found that the ECM produced in cultures grown on the SPS in the presence of the SMFs assembled into a lattice with larger dimensions than the ECM of the cultures grown in the absence of SMFs. During the early stages of the biomineralization process (day 7), the SMF exposed cultures also templated mineral deposition more rapidly than the control cultures. The rapid response is attributed to orientation of diamagnetic ECM proteins already present in the serum, which could then initiate further cellular signaling. SMFs also influenced late stage osteoblast differentiation as measured by the increased rate of osteocalcin secretion and gene expression beginning 15 days after SFM exposure. This correlated with a large increase in mineral deposition, and in cell modulus. GIXD and EDXS analysis confirmed early deposition of crystalline hydroxyapatite. Previous studies on the effects of moderate SMF had focused on cellular gene and protein expression, but did not consider the organization of the ECM fibers. Our ability to form these fibers has allowed us explore this additional effect and highlight its significance in the initiation of the biomineralization process. (C) 2011 Elsevier Ltd. All rights reserved.