Effect of chlorhexidine digluconate on different cell types: A molecular and ultrastructural investigation
Effect of chlorhexidine digluconate on different cell types: A molecular and ultrastructural investigation
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DOI：
10.1016/j.tiv.2007.09.012
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发表时间：
2008-03-01
影响因子：
3.2
通讯作者：
Tani, A.
中科院分区：
文献类型：
--
作者：
Giannelli, M.;Chellini, F.;Tani, A.
尽管已有研究表明二葡萄糖酸氯己定(CHX)对牙周病原体具有杀菌活性，并对牙周组织产生毒性作用，但很少有人研究其对牙周组织的不良作用机制。因此，本研究的目的是研究CHX对牙周炎和种植体周围炎手术治疗中常见靶点细胞的体外细胞毒性作用，并阐明其作用机制。同时对线粒体膜电位、细胞内钙离子动员和活性氧(ROS)生成进行分析，将CHX诱导的细胞损伤与细胞内稳态的关键参数改变相关联。CHX以剂量和时间依赖的方式影响细胞活力，尤其是成骨细胞。其毒性作用包括诱导细胞凋亡和自噬/坏死细胞死亡，线粒体功能紊乱，细胞内钙离子升高和氧化应激。这些数据表明，CHX在体外具有高度的细胞毒性，因此在口腔外科手术中应更加谨慎地使用该防腐剂。(C)2007爱思唯尔有限公司。保留所有权利。
Although several studies have shown that chlorhexidine digluconate (CHX) has bactericidal activity against periodontal pathogens and exerts toxic effects on periodontal tissues, few have been directed to evaluate the mechanisms underlying its adverse effects on these tissues. Therefore, the aim of the present study was to investigate the in vitro cytotoxicity of CHX on cells that could represent common targets for its action in the surgical procedures for the treatment of periodontitis and peri-implantitis and to elucidate its mechanisms of action.Osteoblastic, endothelial and fibroblastic cell lines were exposed to various concentrations of CHX for different times and assayed for cell viability and cell death. Also analysis of mitochondrial membrane potential, intracellular Ca2+ mobilization and reactive oxygen species (ROS) generation were done in parallel, to correlate CHX-induced cell damage with alterations in key parameters of cell homeostasis. CHX affected cell viability in a dose and time-dependent manners, particularly in osteoblasts. Its toxic effect consisted in the induction of apoptotic and autophagic/necrotic cell deaths and involved disturbance of mitochondrial function, intracellular Ca2+ increase and oxidative stress.These data suggest that CHX is highly cytotoxic in vitro and invite to a more cautioned use of the antiseptic in the oral surgical procedures. (C) 2007 Elsevier Ltd. All rights reserved.