The biochemical origins of the surface-enhanced Raman spectra of bacteria: a metabolomics profiling by SERS.
The biochemical origins of the surface-enhanced Raman spectra of bacteria: a metabolomics profiling by SERS.
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DOI：
10.1007/s00216-016-9540-x
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发表时间：
2016-07
影响因子：
4.3
通讯作者：
Ziegler LD
中科院分区：
文献类型：
--
作者：
Premasiri WR;Lee JC;Sauer-Budge A;Théberge R;Costello CE;Ziegler LD
细菌785 nm激发表面增强拉曼散射光谱的主要分子物种是嘌呤降解的代谢物：腺嘌呤、次黄嘌呤、黄嘌呤、鸟嘌呤、尿酸和AMP。这些分子是细菌细胞在营养丰富的环境中浓缩后，在纯净水中产生饥饿反应的结果。利用同位素标记引起的振动位移、细菌SERS光谱的拟合、细菌上清液的SERS和质谱分析、确定的细菌突变体的SERS谱以及SERS谱的酶底物依赖性来鉴定这些分子成分。在这些生物已知的嘌呤代谢途径中，不同的降解/回收酶的存在或不存在，在确定这些基于嘌呤的SERS特征的细菌特异性方面起着核心作用。这些结果为SERS作为一种快速细菌诊断方法的发展提供了生化基础，并说明了SERS如何作为细胞活动的探针更广泛地应用于代谢谱分析。
The dominant molecular species contributing to the 785 nm excited SERS spectra of bacteria are the metabolites of purine degradation: adenine, hypoxanthine, xanthine, guanine, uric acid and AMP. These molecules result from the starvation response of the bacterial cells in pure water washes following enrichment from nutrient rich environments. Vibrational shifts due to isotopic labeling, bacterial SERS spectral fitting, SERS and mass spectrometry analysis of bacterial supernatant, SERS spectra of defined bacterial mutants, and the enzymatic substrate dependence of SERS spectra are used to identify these molecular components. The absence or presence of different degradation/salvage enzymes in the known purine metabolism pathways of these organisms plays a central role in determining the bacterial specificity of these purine-base SERS signatures. These results provide the biochemical basis for the development of SERS as a rapid bacterial diagnostic and illustrate how SERS can be applied more generally for metabolic profiling as a probe of cellular activity.