Enhanced matrix-degrading proteolytic activity within the thin thrombus-covered wall of human abdominal aortic aneurysms
Enhanced matrix-degrading proteolytic activity within the thin thrombus-covered wall of human abdominal aortic aneurysms
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DOI：
10.1016/j.atherosclerosis.2010.04.033
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发表时间：
2010-09-01
期刊：
影响因子：
5.3
通讯作者：
Gutowski, Piotr
中科院分区：
文献类型：
--
作者：
Wiernicki, Ireneusz;Stachowska, Ewa;Gutowski, Piotr
目的：维持动脉弹性蛋白的完整性对于预防腹主动脉瘤（AAA）的发生至关重要。到目前为止，腔内血栓（ILT）厚度对AAA壁内弹性蛋白溶解活性的影响尚未研究。在本研究中的假设，薄血栓与增强蛋白水解活性在人AAA wallowedinvestigated.Methods：标本分析，从两个薄（= 25毫米）血栓覆盖的墙壁，已采取从40例进行择期修复AAA。用酶法测定中性粒细胞弹性蛋白酶活性。采用ELISA法测定活性基质金属蛋白酶-9（MMP-9）、总基质金属蛋白酶-8（MMP-8）和基质金属蛋白酶组织抑制剂-1（TIMP-1）的浓度。结果：弹性蛋白酶活性（P < 0.0001）、活性MMP-9（P = 0.001）、总MMP-8（P <0.0001）和活性MMP-9/总TIMP-1比值（P = 0.002）在血栓覆盖的薄壁组织中均显著高于对照组（P <0.001）。此外，TIMP-1在血栓覆盖的薄壁中的浓度低于血栓覆盖的厚壁（P = 0.003）。在AAA壁部位的薄血栓和厚血栓中，弹性蛋白酶、TIMP-1、MMP-9/TIMP-1比值的测量值之间存在显著正相关，MMP-8的测量值之间存在临界相关。活性MMP-9浓度之间没有相关性sites.Conclusion：目前的研究表明，分化的蛋白酶活性在同一AAA壁和增强内薄血栓覆盖的动脉瘤壁。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Objective: The maintenance of an arterial elastin's integrity is essential in the prevention of abdominal aortic aneurysm (AAA) development. So far, the effect of intraluminal thrombus (ILT) thickness on the elastolytic activity within the AAA wall has not been studied. In the present study the hypothesis that thin thrombus is associated with enhanced proteolytic activity within human AAA wall was investigated.Methods: The specimens for analysis, from both thin (= 25 mm) thrombus-covered wall, had been taken from 40 patients undergoing elective repair of AAA. Weevaluated neutrophil elastase activity with the enzymatic assay. Concentrations of active matrix metalloproteinase-9 (MMP-9), total matrix metalloproteinase-8 (MMP-8), and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) were measured by ELISA. Biochemical parameters were compared with the Wilcoxon signed-rank test.Results: Statistical analysis showed that the activity of elastase (P < 0.0001) as well as concentrations of active MMP-9 (P = 0.001), total MMP-8 (P < 0.0001) and active MMP-9/total TIMP-1 ratio (P = 0.002) were significantly higher in the thin thrombus-covered wall. Furthermore the TIMP-1 was found to have a lower concentration in the thin thrombus-covered in comparison with the thick thrombus-covered wall (P = 0.003). There was a significant positive correlation between measurements in AAA wall sites with thin and thick thrombus for elastase, TIMP-1, MMP-9/TIMP-1 ratio, and a borderline correlation was observed for MMP-8. Active MMP-9 concentration did not correlate between sites.Conclusion: The current study demonstrates the differentiation of protease activity within the same AAA wall and its enhancement within the thin thrombus-covered aneurysm wall. (C) 2010 Elsevier Ireland Ltd. All rights reserved.